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Calpain 1, Large Subunit (CAPN1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002191
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MBS2002191 is an antibody targeting CAPN1. Also known as Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1). Reactive in Mouse, Human, Rat. Host: Rabbit. More details

$210 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Calpain 1, Large Subunit (CAPN1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1)
Product Gene Name
anti-CAPN1 antibody
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF- YPAT FWVNPQFKIR LEEVDDADDY DNRESGCSFL LALMQKHRRR ERRFGRDMET IGFAVYQVPR ELAGQPVHLK RDFFLANASR AQSEHFINLR EVSNRIRLPP GEYIVVPSTF EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE EIDDNFKTLF SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL NKKLHELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKL LDTD
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Human, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
0.42mg/ml (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against CAPN1. It has been selected for its ability to recognize CAPN1 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: CAPN1 (MBS2002191)
Immunogen: CAPN1 (MBS2009975)
Matching Pairs
Unconjugated Antibody: CAPN1 (MBS2002191)
APC-CY7 Conjugated Antibody: CAPN1 (MBS2061144)
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Molecular Weight
82,106 Da
3D Structure
ModBase 3D Structure for O35350
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant CAPN1 (Tyr387~Asp694) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2061148
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-CAPN1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-CAPN1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-CAPN1 antibody

Western Blot: Sample: Lane1: Mouse Serum; Lane2: Mouse Placenta Tissue.
Rabbit Anti-CAPN1 Polyclonal Antibody – Cat# MBS2002191

Western Blot (WB) of anti-CAPN1 antibody

Western Blot: Sample: Recombinant CAPN1, Mouse.
Rabbit Anti-CAPN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002191

Immunohistochemistry (IHC) of anti-CAPN1 antibody

DAB staining on IHC-P; Samples: Mouse Liver Tissue.
Rabbit Anti-CAPN1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002191

Immunohistochemistry (IHC) of anti-CAPN1 antibody

DAB staining on IHC-P; Samples: Mouse Ovary Tissue
Rabbit Anti-CAPN1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002191

Immunohistochemistry (IHC) of anti-CAPN1 antibody

DAB staining on IHC-P; Samples: Mouse Testis Tissue.
Rabbit Anti-CAPN1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002191

Other Notes

Small volumes of anti-CAPN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CAPN1. It may not necessarily be applicable to this product.

NCBI GI #
160333229
NCBI GeneID
12333
NCBI Accession #
NP_001103974.1
UniProt Primary Accession #
O35350
UniProt Secondary Accession #
O88666
UniProt Related Accession #
O35350
GenBank Nucleotide #
NM_001110504.1
NCBI Official Full Name
calpain-1 catalytic subunit
NCBI Official Synonym Full Names
calpain 1
NCBI Official Symbol
Capn1
NCBI Official Synonym Symbols
Capa1; Capa-1; mu-calpin
UniProt Protein Name
Calpain-1 catalytic subunit
UniProt Synonym Protein Names
Calcium-activated neutral proteinase 1
Protein Family
Calpain
UniProt Gene Name
Capn1
UniProt Synonym Gene Names
muCANP
NCBI Protein Information
calpain-1 catalytic subunit

UniProt Comments for CAPN1

CAPN1: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family.

Protein type: EC 3.4.22.52; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 19 A|19 4.34 cM

Cellular Component: cytoplasm; cytosol; focal adhesion; intracellular; lysosome; membrane; mitochondrion; plasma membrane

Molecular Function: calcium ion binding; calcium-dependent cysteine-type endopeptidase activity; cysteine-type peptidase activity; cytoskeletal protein binding; hydrolase activity; metal ion binding; peptidase activity; protein binding

Biological Process: mammary gland involution; protein autoprocessing; proteolysis; receptor catabolic process; regulation of catalytic activity
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Research Articles on CAPN1

1. mutations in CAPN1 are an additional cause of ataxia in mammals, including humans.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CAPN1 antibody

 Reference Product  PubMed Publications
 Cast antibody  >37 publications with CAPN1 and Cast
 Capns1 antibody  >18 publications with CAPN1 and Capns1
 Ptpn1 antibody  >3 publications with CAPN1 and Ptpn1
 Bax antibody  >1 publications with CAPN1 and Bax
 Tln1 antibody  >1 publications with CAPN1 and Tln1
 Casp12 antibody  >1 publications with CAPN1 and Casp12

Pathways associated with anti-CAPN1 antibody

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 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
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 Apoptosis Signaling Pathway antibodies  Apoptosis Signaling Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Deregulated CDK5 Triggers Multiple Neurodegenerative Pathways In Alzheimer's Disease Models antibodies  Deregulated CDK5 Triggers Multiple Neurodegenerative Pathways In Alzheimer's Disease Models Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram

Diseases associated with anti-CAPN1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >27 publications with CAPN1 and Nervous System Diseases
 Heart Diseases Antibodies  >7 publications with CAPN1 and Heart Diseases
 Nerve Degeneration Antibodies  >5 publications with CAPN1 and Nerve Degeneration
 Inflammation Antibodies  >5 publications with CAPN1 and Inflammation
 Breast Neoplasms Antibodies  >5 publications with CAPN1 and Breast Neoplasms
 Necrosis Antibodies  >5 publications with CAPN1 and Necrosis
 Fibrosis Antibodies  >4 publications with CAPN1 and Fibrosis
 Cardiomyopathies Antibodies  >4 publications with CAPN1 and Cardiomyopathies
 Kidney Diseases Antibodies  >3 publications with CAPN1 and Kidney Diseases
 Weight Gain Antibodies  >3 publications with CAPN1 and Weight Gain

Organs/Tissues associated with anti-CAPN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >56 publications with CAPN1 and Muscle
 Blood Antibodies  >27 publications with CAPN1 and Blood
 Brain Antibodies  >23 publications with CAPN1 and Brain
 Eye Antibodies  >11 publications with CAPN1 and Eye
 Adipose Tissue Antibodies  >9 publications with CAPN1 and Adipose Tissue
 Kidney Antibodies  >7 publications with CAPN1 and Kidney
 Skin Antibodies  >7 publications with CAPN1 and Skin
 Lung Antibodies  >6 publications with CAPN1 and Lung
 Bone Antibodies  >6 publications with CAPN1 and Bone
 Connective Tissue Antibodies  >6 publications with CAPN1 and Connective Tissue
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