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KLK3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200221

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. PSA is present in small quantities in the serum of… More details

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Product Overview

Online Datasheet in PDF
Product Name
KLK3, Monoclonal Antibody
Full Product Name
KLK3 antibody
Product Gene Name
Product Synonym Names
Prostate-specific antigen; APS; hK3; KLK2A1; PSA
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT1D10
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
27,895 Da
OMIM
M30704 mRNA
3D Structure
ModBase 3D Structure for P15514
Antigen Species
Human
Immunogen
Recombinant human KLK3 (25-261aa) purified from E. coli
Clone Note
Anti-human KLK3 mAb, clone AT1D10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KLK3 protein.

Product Categories/Family for anti-KLK3 antibody

Applications Tested/Suitable for anti-KLK3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-KLK3 antibody
WB: 1:500 ~ 1:5000.

Western Blot (WB) of anti-KLK3 antibody

The extracts of PC-3 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLK3 antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-KLK3 Monoclonal Antibody – Cat# MBS200221

Immunofluorescence (IF) of anti-KLK3 antibody

ICC/IF analysis of KLK3 in LNCap cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KLK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-KLK3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200221

Product References and Citations for anti-KLK3 antibody

Balk SP, Ko YJ, Bubley GJ (January 2003). J. Clin. Oncol. 21 (2): 383-91.; Lilja H (November 2003). Urology 62 (5 Suppl 1): 27-33.; Rao AR, Motiwala HG, Karim OM (January 2008). BJU Int. 101 (1): 5-10.

Other Notes

Small volumes of anti-KLK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLK3 purchase

MBS200221 is an antibody targeting KLK3. Also known as KLK3 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KLK3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
amphiregulin preproprotein
NCBI Official Synonym Full Names
amphiregulin
NCBI Official Symbol
AREG
NCBI Official Synonym Symbols
AR; SDGF; AREGB; CRDGF
UniProt Protein Name
Amphiregulin
UniProt Synonym Protein Names
Colorectum cell-derived growth factor
Protein Family
UniProt Gene Name
AREG
UniProt Synonym Gene Names
CRDGF
NCBI Protein Information
amphiregulin

NCBI Summary for KLK3

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]
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UniProt Comments for KLK3

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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