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Neurofilament, Light Polypeptide (NEFL) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002250
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MBS2002250 is an antibody targeting NEFL. Also known as Biotin-Linked Antibody to Neurofilament, Light Polypeptide (NEFL). Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Neurofilament, Light Polypeptide (NEFL), Polyclonal Antibody
Full Product Name
Biotin-Linked Antibody to Neurofilament, Light Polypeptide (NEFL)
Product Gene Name
anti-NEFL antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, 50% glycerol 0.05% Proclin-300.
Purity/Purification
Antigen-specific Affinity Chromatography.
Concentration
500ug/ml (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Chromosome Location
Chromosome: 8; NC_000008.10 (24808468..24814383, complement). Location: 8p21
OMIM
162280
3D Structure
ModBase 3D Structure for P07196
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Organism Species
Homo sapiens (Human)
Source
Antibody labeling
Label
Biotin
Unconjugated Antibody
MBS2006101
Traits
Liquid
Cross Reactivity
Human, Mouse, Rat
Immunogen
Recombinant NEFL (Lys281~Glu396) expressed in E.coli. (MBS2009843)

Product Categories/Family for anti-NEFL antibody

Biotin Antibody
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-NEFL antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-NEFL antibody
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.

Other Notes

Small volumes of anti-NEFL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEFL. It may not necessarily be applicable to this product.

NCBI GI #
105990539
NCBI GeneID
4747
NCBI Accession #
NP_006149.2
UniProt Primary Accession #
P07196
UniProt Secondary Accession #
Q16154; Q8IU72; B9ZVN2
UniProt Related Accession #
P07196
GenBank Nucleotide #
NM_006158.4
NCBI Official Full Name
neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament, light polypeptide
NCBI Official Symbol
NEFL
NCBI Official Synonym Symbols
NFL; NF-L; NF68; CMT1F; CMT2E
UniProt Protein Name
Neurofilament light polypeptide
UniProt Synonym Protein Names
68 kDa neurofilament protein; Neurofilament triplet L protein
Protein Family
Neurofilament light polypeptide
UniProt Gene Name
NEFL
UniProt Synonym Gene Names
NF68; NFL; NF-L
UniProt Entry Name
NFL_HUMAN
NCBI Protein Information
neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein

NCBI Summary for NEFL

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
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UniProt Comments for NEFL

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol

Molecular Function: protein C-terminus binding; protein binding, bridging; identical protein binding; protein domain specific binding; protein binding; phospholipase binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; axon regeneration in the peripheral nervous system; retrograde axon cargo transport; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; anterograde axon cargo transport; locomotion; neuromuscular process controlling balance; neurite morphogenesis; response to corticosterone stimulus

Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f
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Research Articles on NEFL

1. In the title.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NEFL antibody

 Reference Product  PubMed Publications
 MTMR2 antibody  >6 publications with NEFL and MTMR2
 NEFH antibody  >3 publications with NEFL and NEFH
 GAN antibody  >2 publications with NEFL and GAN
 NEFM antibody  >2 publications with NEFL and NEFM
 TSC1 antibody  >1 publications with NEFL and TSC1

Pathways associated with anti-NEFL antibody

 Products by Pathway  Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies  CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram
 CREB Phosphorylation Through The Activation Of Ras Pathway antibodies  CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway antibodies  Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway Diagram
 Post NMDA Receptor Activation Events Pathway antibodies  Post NMDA Receptor Activation Events Pathway Diagram
 Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway antibodies  Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway Diagram

Diseases associated with anti-NEFL antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >50 publications with NEFL and Nervous System Diseases
 Peripheral Nervous System Diseases Antibodies  >43 publications with NEFL and Peripheral Nervous System Diseases
 Nervous System Malformations Antibodies  >41 publications with NEFL and Nervous System Malformations
 Polyneuropathies Antibodies  >41 publications with NEFL and Polyneuropathies
 Neoplasms Antibodies  >30 publications with NEFL and Neoplasms
 Breast Neoplasms Antibodies  >7 publications with NEFL and Breast Neoplasms
 Brain Diseases Antibodies  >5 publications with NEFL and Brain Diseases
 Charcot-Marie-Tooth disease, Type 1F Antibodies  >4 publications with NEFL and Charcot-Marie-Tooth disease, Type 1F
 Pain Antibodies  >3 publications with NEFL and Pain
 Lung Neoplasms Antibodies  >2 publications with NEFL and Lung Neoplasms

Organs/Tissues associated with anti-NEFL antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >32 publications with NEFL and Nerve
 Brain Antibodies  >16 publications with NEFL and Brain
 Prostate Antibodies  >11 publications with NEFL and Prostate
 Spinal Cord Antibodies  >6 publications with NEFL and Spinal Cord
 Lung Antibodies  >4 publications with NEFL and Lung
 Eye Antibodies  >3 publications with NEFL and Eye
 Ganglia Antibodies  >2 publications with NEFL and Ganglia
 Kidney Antibodies  >2 publications with NEFL and Kidney
 Liver Antibodies  >1 publications with NEFL and Liver
 Bone Marrow Antibodies  >1 publications with NEFL and Bone Marrow
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