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TP53I3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200228

TP53I3 participates in the generation of reactive oxygen species (ROS). TP53I3 has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. TP53I3 has low NADPH-dependent diamine reductase activity… More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TP53I3, Monoclonal Antibody
Full Product Name
TP53I3 antibody
Product Gene Name
anti-TP53I3 antibody
Product Synonym Names
Quinone oxidoreductase PIG3; PIG3; Quinone oxidoreductase PIG3 p53 induced gene 3 protein; Putative quinone oxidoreductase; quinone oxidoreductase homolog; TP53I3; Tumor protein p53 inducible protein 3.
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT1C9
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
25,402 Da
OMIM
AF010309 mRNA
3D Structure
ModBase 3D Structure for Q53FA7
Antigen Species
Human
Immunogen
Recombinant human TP53I3 (1-332aa) purified from E. coli
Clone Note
Anti-human TP53I3 mAb, clone AT1C9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TP53I3 protein.

Product Categories/Family for anti-TP53I3 antibody

Cancer
Applications Tested/Suitable for anti-TP53I3 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-TP53I3 antibody
WB: 1:3,000.

Western Blot (WB) of anti-TP53I3 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TP53I3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : SW480 cell lysate The Cell lysates (1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TP53I3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: TP53I3 Transfected 293T cell lysate
Mouse Anti-TP53I3 Monoclonal Antibody – Cat# MBS200228

Immunofluorescence (IF) of anti-TP53I3 antibody

ICC/IF analysis of TP53I3 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TP53I3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-TP53I3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200228

Product References and Citations for anti-TP53I3 antibody

Porte S, et al. (2009) J Biol Chem.. 284 (25):17194-205 ; Flatt PM. et al. (2000) Cancer Lett. 156 (1):63-72.; J Biol Chem. 2009 Jun 19;284 (25):17194-205.

Other Notes

Small volumes of anti-TP53I3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TP53I3 purchase

MBS200228 is an antibody targeting TP53I3. Also known as TP53I3 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TP53I3. It may not necessarily be applicable to this product.

NCBI GI #
22538446
NCBI GeneID
9540
NCBI Accession #
NP_671713
UniProt Primary Accession #
Q53FA7
UniProt Secondary Accession #
O14679; O14685; Q38G78; Q6JLE7; Q9BWB8; D6W533
UniProt Related Accession #
Q53FA7
GenBank Nucleotide #
NM_147184.3
NCBI Official Full Name
quinone oxidoreductase PIG3 isoform 1
NCBI Official Synonym Full Names
tumor protein p53 inducible protein 3
NCBI Official Symbol
TP53I3
NCBI Official Synonym Symbols
PIG3
UniProt Protein Name
Quinone oxidoreductase PIG3
UniProt Synonym Protein Names
Tumor protein p53-inducible protein 3; p53-induced gene 3 protein
Protein Family
Quinone oxidoreductase
UniProt Gene Name
TP53I3
UniProt Synonym Gene Names
PIG3
NCBI Protein Information
quinone oxidoreductase PIG3

NCBI Summary for TP53I3

The protein encoded by this gene is similar to oxidoreductases, which are enzymes involved in cellular responses to oxidative stresses and irradiation. This gene is induced by the tumor suppressor p53 and is thought to be involved in p53-mediated cell death. It contains a p53 consensus binding site in its promoter region and a downstream pentanucleotide microsatellite sequence. P53 has been shown to transcriptionally activate this gene by interacting with the downstream pentanucleotide microsatellite sequence. The microsatellite is polymorphic, with a varying number of pentanucleotide repeats directly correlated with the extent of transcriptional activation by p53. It has been suggested that the microsatellite polymorphism may be associated with differential susceptibility to cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
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UniProt Comments for TP53I3

May be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent beta-naphthoquinone reductase activity, with a preference for 1,2-beta-naphthoquinone over 1,4-beta-naphthoquinone. Has low NADPH-dependent diamine reductase activity (in vitro).

Research Articles on TP53I3

1. Results revealed that PIG3 expression levels positively correlated with poor prognosis of non-small cell lung cancer (NSCLC) patients and indicate that PIG3 promotes NSCLC progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TP53I3 antibody

 Reference Product  PubMed Publications
 TP53 antibody  >14 publications with TP53I3 and TP53
 TP63 antibody  >1 publications with TP53I3 and TP63

Pathways associated with anti-TP53I3 antibody

 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 TP53 Regulates Transcription Of Cell Death Genes Pathway antibodies  TP53 Regulates Transcription Of Cell Death Genes Pathway Diagram
 TP53 Regulates Transcription Of Several Additional Cell Death Genes Whose Specific Roles In P53-dependent Apoptosis Remain Uncertain Pathway antibodies  TP53 Regulates Transcription Of Several Additional Cell Death Genes Whose Specific Roles In P53-dependent Apoptosis Remain Uncertain Pathway Diagram
 Transcriptional Regulation By TP53 Pathway antibodies  Transcriptional Regulation By TP53 Pathway Diagram
 P53 Signaling Pathway antibodies  P53 Signaling Pathway Diagram
 P53 Signaling Pathway antibodies  P53 Signaling Pathway Diagram

Diseases associated with anti-TP53I3 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >15 publications with TP53I3 and Carcinoma
 Lung Neoplasms Antibodies  >7 publications with TP53I3 and Lung Neoplasms
 Breast Neoplasms Antibodies  >5 publications with TP53I3 and Breast Neoplasms
 Adenocarcinoma Antibodies  >4 publications with TP53I3 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >3 publications with TP53I3 and Cell Transformation, Neoplastic
 Carcinoma, Hepatocellular Antibodies  >3 publications with TP53I3 and Carcinoma, Hepatocellular
 Carcinoma, Squamous Cell Antibodies  >2 publications with TP53I3 and Carcinoma, Squamous Cell
 Colonic Neoplasms Antibodies  >2 publications with TP53I3 and Colonic Neoplasms
 Nervous System Diseases Antibodies  >1 publications with TP53I3 and Nervous System Diseases
 Liver Diseases Antibodies  >1 publications with TP53I3 and Liver Diseases

Organs/Tissues associated with anti-TP53I3 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >7 publications with TP53I3 and Lung
 Blood Antibodies  >3 publications with TP53I3 and Blood
 Liver Antibodies  >3 publications with TP53I3 and Liver
 Bone Antibodies  >3 publications with TP53I3 and Bone
 Stomach Antibodies  >2 publications with TP53I3 and Stomach
 Skin Antibodies  >2 publications with TP53I3 and Skin
 Vascular Antibodies  >2 publications with TP53I3 and Vascular
 Brain Antibodies  >2 publications with TP53I3 and Brain
 Nerve Antibodies  >1 publications with TP53I3 and Nerve
 Kidney Antibodies  >1 publications with TP53I3 and Kidney
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