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Plasminogen Activator Inhibitor 1 (PAI1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002302
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MBS2002302 is an antibody targeting PAI1. Also known as Polyclonal Antibody to Plasminogen Activator Inhibitor 1 (PAI1). Reactive in Human. Host: Rabbit. More details

$190 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Plasminogen Activator Inhibitor 1 (PAI1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Plasminogen Activator Inhibitor 1 (PAI1)
Product Gene Name
anti-PAI1 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
0.5mg/ml (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: PAI1 (MBS2002302)
Biotin Conjugated Antibody: PAI1 (MBS2003656)
Matching Pairs
Unconjugated Antibody: PAI1 (MBS2002302)
Immunogen: PAI1 (MBS2010253)
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OMIM
X04429 mRNA
3D Structure
ModBase 3D Structure for P05121
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant PAI1 (His25~Pro402) expressed in E.coli (MBS2010253).
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PAI1 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP)
Application Notes for anti-PAI1 antibody
Western blotting: 0.01-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-PAI1 antibody

Western Blot:
Sample: Recombinant PAI1, Human.
Rabbit Anti-PAI1 Polyclonal Antibody – Cat# MBS2002302

Immunohistochemistry (IHC) of anti-PAI1 antibody

DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human PAI1 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047)
Rabbit Anti-PAI1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002302

Immunohistochemistry (IHC) of anti-PAI1 antibody

DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human PAI1 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-PAI1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002302

Immunohistochemistry (IHC) of anti-PAI1 antibody

DAB staining on fromalin fixed paraffin-embedded Kidney tissue
Rabbit Anti-PAI1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002302

Other Notes

Small volumes of anti-PAI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PAI1. It may not necessarily be applicable to this product.

NCBI GI #
10835159
NCBI GeneID
5054
NCBI Accession #
NP_000593.1
UniProt Primary Accession #
P05121
UniProt Secondary Accession #
B7Z4S0; F8WD53
UniProt Related Accession #
P05121
GenBank Nucleotide #
NM_000602.4
NCBI Official Full Name
plasminogen activator inhibitor 1
NCBI Official Synonym Full Names
serpin family E member 1
NCBI Official Symbol
SERPINE1
NCBI Official Synonym Symbols
PAI; PAI1; PAI-1; PLANH1
UniProt Protein Name
Plasminogen activator inhibitor 1
UniProt Synonym Protein Names
Endothelial plasminogen activator inhibitor; Serpin E1
UniProt Gene Name
SERPINE1
UniProt Synonym Gene Names
PAI1; PLANH1; PAI; PAI-1
NCBI Protein Information
plasminogen activator inhibitor 1

NCBI Summary for PAI1

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
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UniProt Comments for PAI1

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular region; extracellular space; plasma membrane

Molecular Function: protease binding; protein binding; receptor binding; serine-type endopeptidase inhibitor activity

Biological Process: angiogenesis; chronological cell aging; circadian rhythm; defense response to Gram-negative bacterium; extracellular matrix organization; fibrinolysis; negative regulation of blood coagulation; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; negative regulation of fibrinolysis; negative regulation of smooth muscle cell migration; platelet degranulation; positive regulation of angiogenesis; positive regulation of blood coagulation; positive regulation of inflammatory response; positive regulation of interleukin-8 production; positive regulation of receptor-mediated endocytosis; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity

Disease: Plasminogen Activator Inhibitor-1 Deficiency
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Research Articles on PAI1

1. Study documented higher serum PAI-1 in HCV-induced hepatocellular carcinoma than viral infection-free controls, but interestingly, lower than HCV-induced liver cirrhosis patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PAI1 antibody

 Reference Product  PubMed Publications
 TGFB1 antibody  >480 publications with PAI1 and TGFB1
 SMAD4 antibody  >77 publications with PAI1 and SMAD4
 EGF antibody  >60 publications with PAI1 and EGF
 HIF1A antibody  >59 publications with PAI1 and HIF1A
 PLG antibody  >42 publications with PAI1 and PLG
 PLAT antibody  >22 publications with PAI1 and PLAT
 PLAU antibody  >11 publications with PAI1 and PLAU
 VTN antibody  >5 publications with PAI1 and VTN
 ALB antibody  >3 publications with PAI1 and ALB

Pathways associated with anti-PAI1 antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway antibodies  BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram

Diseases associated with anti-PAI1 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3005 publications with PAI1 and Cardiovascular Diseases
 Neoplasms Antibodies  >1344 publications with PAI1 and Neoplasms
 Thrombosis Antibodies  >1312 publications with PAI1 and Thrombosis
 Inflammation Antibodies  >1166 publications with PAI1 and Inflammation
 Heart Diseases Antibodies  >997 publications with PAI1 and Heart Diseases
 Necrosis Antibodies  >788 publications with PAI1 and Necrosis
 Atherosclerosis Antibodies  >731 publications with PAI1 and Atherosclerosis
 Hypertension Antibodies  >653 publications with PAI1 and Hypertension
 Fibrosis Antibodies  >614 publications with PAI1 and Fibrosis
 Kidney Diseases Antibodies  >492 publications with PAI1 and Kidney Diseases

Organs/Tissues associated with anti-PAI1 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >2660 publications with PAI1 and Vascular
 Liver Antibodies  >554 publications with PAI1 and Liver
 Lung Antibodies  >529 publications with PAI1 and Lung
 Embryonic Tissue Antibodies  >322 publications with PAI1 and Embryonic Tissue
 Umbilical Cord Antibodies  >206 publications with PAI1 and Umbilical Cord
 Skin Antibodies  >181 publications with PAI1 and Skin
 Placenta Antibodies  >121 publications with PAI1 and Placenta
 Intestine Antibodies  >67 publications with PAI1 and Intestine
 Pancreas Antibodies  >39 publications with PAI1 and Pancreas
 Amniotic Fluid Antibodies  >12 publications with PAI1 and Amniotic Fluid
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