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Osteonectin (ON) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002406
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2002406 is an antibody targeting ON. Also known as Polyclonal Antibody to Osteonectin (ON). Reactive in Rat. Host: Rabbit. More details

$210 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Osteonectin (ON), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Osteonectin (ON)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSG S-APQ TEAAEEMVAE ETVVEETGLP VGANPVQVEM GEFEEGAEET VEEVVAENPC QNHHCKHGKV CELDESNTPM CVCQDPTSCP APIGEFEKVC SNDNKTFDSS CHFFATKCTL EGTKKGHKLH LDYIGPCKYI APCLDSELTE FPLRMRDWLK NVLVTLYERD EGNNLLTEKQ KLRVKKIHEN EKRLEAGDHP VELLARDFEK NYNMYIFPVH WQFGQLDQHP IDGYLSHTEL APLRAPLIPM EHCTTRFFET CDLDNDKYIA LEEWAGCFGI KEQDINKDLV I
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against ON. It has been selected for its ability to recognize ON in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: ON (MBS2002406)
Biotin Conjugated Antibody: ON (MBS2003129)
Matching Pairs
Unconjugated Antibody: ON (MBS2002406)
Immunogen: ON (MBS2009453)
Molecular Weight
34,296 Da
3D Structure
ModBase 3D Structure for P16975
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant ON (Ala18~Ile301) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2047511
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-ON antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-ON antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-ON antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-ON Polyclonal Antibody – Cat# MBS2002406

Immunohistochemistry (IHC) of anti-ON antibody

DAB staining on fromalin fixed paraffin-embedded Lung tissue)
Rabbit Anti-ON Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002406

Other Notes

Small volumes of anti-ON antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ON. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
SPARC
NCBI Official Synonym Full Names
secreted protein acidic and cysteine rich
NCBI Official Symbol
Sparc
UniProt Protein Name
SPARC
UniProt Synonym Protein Names
Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
Protein Family
UniProt Gene Name
Sparc
UniProt Synonym Gene Names
BM-40; ON
NCBI Protein Information
SPARC

NCBI Summary for ON

secreted structural protein; may be a component of collagen and the extracellular matrix [RGD, Feb 2006]

UniProt Comments for ON

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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