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GCLM Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200243

GCLM (Glutamate-cysteine ligase, modifier subunit), also known as gamma-glutamylcysteine synthetase, is first rate limiting enzyme of glutathione synthesis. GCLM is heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GCLM, Monoclonal Antibody
Full Product Name
GCLM antibody
Product Gene Name
Product Synonym Names
Glutamate-cysteine ligase modifier subunit; Glutamate-cysteine ligase; modifier subunit; GLCLR
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT2D12
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
28,135 Da
OMIM
L35546 mRNA
3D Structure
ModBase 3D Structure for P48507
Antigen Species
Human
Immunogen
Recombinant human GCLM (1-274aa) purified from E. coli
Clone Note
Anti-human GCLM mAb, clone AT2D12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GCLM protein.

Product Categories/Family for anti-GCLM antibody

Applications Tested/Suitable for anti-GCLM antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-GCLM antibody
WB: 1:3000.

Western Blot (WB) of anti-GCLM antibody

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-GCLM Monoclonal Antibody – Cat# MBS200243

Immunofluorescence (IF) of anti-GCLM antibody

ICC/IF analysis of GCLM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GCLM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-GCLM Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200243

Product References and Citations for anti-GCLM antibody

Dickinson D.A., et al. (2005) Free Radic Biol Med 37 (8): 1152-1159; Inoue Y., et al. (2003) Int J Oncol 23 (5): 1333-1339; Robertson N.G., et al. (1995) Genomics 23 (1): 42-50

Other Notes

Small volumes of anti-GCLM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GCLM purchase

MBS200243 is an antibody targeting GCLM. Also known as GCLM antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GCLM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glutamate--cysteine ligase regulatory subunit isoform 1
NCBI Official Synonym Full Names
glutamate-cysteine ligase modifier subunit
NCBI Official Symbol
GCLM
NCBI Official Synonym Symbols
GLCLR
UniProt Protein Name
Glutamate--cysteine ligase regulatory subunit
UniProt Synonym Protein Names
GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit
UniProt Gene Name
GCLM
UniProt Synonym Gene Names
GLCLR
NCBI Protein Information
glutamate--cysteine ligase regulatory subunit

NCBI Summary for GCLM

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GCLM antibody

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