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Endothelin 1 (EDN1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002432
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MBS2002432 is an antibody targeting EDN1. Also known as Polyclonal Antibody to Endothelin 1 (EDN1). Reactive in Mouse, Rat. Host: Rabbit. More details

$210 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Endothelin 1 (EDN1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Endothelin 1 (EDN1)
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
0.5mg/mL (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: EDN1 (MBS2002432)
Biotin Conjugated Antibody: EDN1 (MBS2001978)
Matching Pairs
Unconjugated Antibody: EDN1 (MBS2002432)
Immunogen: EDN1 (MBS2010595)
3D Structure
ModBase 3D Structure for P22388
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Mouse
Immunogen
Recombinant EDN1 (Ser54~His202) expressed in E.coli ( MBS2010595 ).
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-EDN1 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes for anti-EDN1 antibody
Western blotting: 0.01-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user

Western Blot (WB) of anti-EDN1 antibody

Western Blot: Sample: Rat Serum; Primary Ab: 1ug/ml Rabbit Anti-Rat EDN1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 )
Rabbit Anti-EDN1 Polyclonal Antibody – Cat# MBS2002432

Western Blot (WB) of anti-EDN1 antibody

Figure. Western Blot; Sample: Recombinant EDN1, Rat.
Rabbit Anti-EDN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002432

Western Blot (WB) of anti-EDN1 antibody

Western Blot; Sample: Rat Lung lysate;
Primary Ab: 1ug/ml Rabbit Anti-Rat EDN1 Antibody
Second Ab: 0.2ug/mL HRP-LinkedCaprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047)
Rabbit Anti-EDN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002432

Western Blot (WB) of anti-EDN1 antibody

Western Blot; Sample: Rat Placenta lysate
Primary Ab: 0.1ug/ml Rabbit Anti-Rat EDN1 Antibody
Second Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-EDN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002432

Other Notes

Small volumes of anti-EDN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EDN1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
endothelin-1 preproprotein
NCBI Official Synonym Full Names
endothelin 1
NCBI Official Symbol
Edn1
NCBI Official Synonym Symbols
Et1
UniProt Protein Name
Endothelin-1
UniProt Synonym Protein Names
Preproendothelin-1; PPET1
Protein Family
UniProt Gene Name
Edn1
UniProt Synonym Gene Names
ET-1; PPET1
NCBI Protein Information
endothelin-1

NCBI Summary for EDN1

activates ERK1/2 mediated signaling; may play a role in regulation of cardiac contractile force [RGD, Feb 2006]

UniProt Comments for EDN1

Endothelins are endothelium-derived vasoconstrictor peptides.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EDN1 antibody

Organs/Tissues associated with anti-EDN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >17 publications with EDN1 and Lung
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