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PPM1F Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200255

PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block… More details

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Product Overview

Online Datasheet in PDF
Product Name
PPM1F, Monoclonal Antibody
Full Product Name
PPM1F antibody
Product Gene Name
Product Synonym Names
Protein phosphatase 1F; CAMKP; CaMKPase; FEM-2; hFEM-2; POPX2
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT6D11
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
38,518 Da
3D Structure
ModBase 3D Structure for P49593
Antigen Species
Human
Immunogen
Recombinant human PPM1F (1-454aa) purified from E. coli
Clone Note
Anti-human PPM1F mAb, clone AT6D11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPM1F protein.

Product Categories/Family for anti-PPM1F antibody

Applications Tested/Suitable for anti-PPM1F antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-PPM1F antibody
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-PPM1F antibody

The lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (2ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PPM1F Transfected 293T cell lysate
Mouse Anti-PPM1F Monoclonal Antibody – Cat# MBS200255

Immunofluorescence (IF) of anti-PPM1F antibody

ICC/IF analysis of PPM1F in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1F antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-PPM1F Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200255

Product References and Citations for anti-PPM1F antibody

Koh, CG., et al. (2002) Curr Biol 12 (4): 317-21; Tan, KM., et al. (2001) J Biol Chem 276 (47): 44193-202

Other Notes

Small volumes of anti-PPM1F antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPM1F purchase

MBS200255 is an antibody targeting PPM1F. Also known as PPM1F antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PPM1F. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein phosphatase 1F
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1F
NCBI Official Symbol
PPM1F
NCBI Official Synonym Symbols
CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase
UniProt Protein Name
Protein phosphatase 1F
UniProt Synonym Protein Names
Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; CaMKPase; Partner of PIX 2; Protein fem-2 homolog; hFem-2
Protein Family
UniProt Gene Name
PPM1F
UniProt Synonym Gene Names
KIAA0015; POPX2; CaM-kinase phosphatase; CaMKPase; hFem-2
NCBI Protein Information
protein phosphatase 1F

NCBI Summary for PPM1F

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for PPM1F

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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