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Homogentisate-1,2-Dioxygenase (HGD) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002753
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MBS2002753 is an antibody targeting HGD. Also known as Polyclonal Antibody to Homogentisate-1,2-Dioxygenase (HGD). Reactive in Human, Mouse, Rat, Pig. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Homogentisate-1,2-Dioxygenase (HGD), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Homogentisate-1,2-Dioxygenase (HGD)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF- SGF GNECSSEDPR CPGSLPEGQN NPQVCPYNLY AEQLSGSAFT CPRSTNKRSW LYRILPSVSH KPFESIDEGQ VTHNWDEVDP DPNQLRWKPF EIPKASQKKV DFVSGLHTLC GAGDIKSNNG LAIHIFLCNT SMENRCFYNS DGDFLIVPQK GNLLIYTEFG KMLVQPNEIC VIQRGMRFSI DVFEETRGYI LEVYG
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Pig
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against HGD. It has been selected for its ability to recognize HGD in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: HGD (MBS2002753)
Biotin Conjugated Antibody: HGD (MBS2007102)
Matching Pairs
Unconjugated Antibody: HGD (MBS2002753)
Immunogen: HGD (MBS2009467)
Molecular Weight
49,964 Da
OMIM
U63008 mRNA
3D Structure
ModBase 3D Structure for Q93099
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant HGD (Ser8~Gly205) expressed in E.coli.
Cross Reactivity
Human, Pig
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074249
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-HGD antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-HGD antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-HGD antibody

Western Blot: Sample: Porcine Liver lysate; Primary Ab: 2ug/ml Rabbit Anti-Human HGD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-HGD Polyclonal Antibody – Cat# MBS2002753

Western Blot (WB) of anti-HGD antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-HGD Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002753

Immunohistochemistry (IHC) of anti-HGD antibody

DAB staining on IHC-P. Samples: Human Tissue)
Rabbit Anti-HGD Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002753

Other Notes

Small volumes of anti-HGD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HGD. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
homogentisate 1,2-dioxygenase
NCBI Official Synonym Full Names
homogentisate 1,2-dioxygenase
NCBI Official Symbol
HGD
NCBI Official Synonym Symbols
AKU; HGO
UniProt Protein Name
Homogentisate 1,2-dioxygenase
UniProt Synonym Protein Names
Homogentisate oxygenase; Homogentisic acid oxidase; Homogentisicase
UniProt Gene Name
HGD
UniProt Synonym Gene Names
HGO
NCBI Protein Information
homogentisate 1,2-dioxygenase

NCBI Summary for HGD

This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.[provided by RefSeq, May 2010]

UniProt Comments for HGD

HGD: Defects in HGD are the cause of alkaptonuria (AKU). AKU is an autosomal recessive error of metabolism characterized by an increase in the level of homogentisic acid. The clinical manifestations of AKU are urine that turns dark on standing and alkalinization, black ochronotic pigmentation of cartilage and collagenous tissues, and spine arthritis. Belongs to the homogentisate dioxygenase family.

Protein type: Amino Acid Metabolism - tyrosine; EC 1.13.11.5; Oxidoreductase

Chromosomal Location of Human Ortholog: 3q13.33

Cellular Component: cytosol

Molecular Function: homogentisate 1,2-dioxygenase activity; identical protein binding; metal ion binding; protein binding

Biological Process: L-phenylalanine catabolic process; tyrosine catabolic process

Disease: Alkaptonuria
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HGD antibody

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