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Diamine Oxidase (DAO) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002759
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2002759 is an antibody targeting DAO. Also known as Polyclonal Antibody to Diamine Oxidase (DAO). Reactive in Mouse, Rat. Host: Rabbit. More details

$205 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Diamine Oxidase (DAO), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Diamine Oxidase (DAO)
Product Gene Name
anti-DAO antibody
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-HTPVTV AGP HVVQPSGPRY KLEGNVVLYG DWSFSYRLRS SSGLQIFNVL FGGERVAYEV SVQEAVALYG GHTPAGMQTK YIDVGWGLGS VTHELAPGID CPETATFLDA FHYYDSDGPV LYPRALCLFE MPTGVPLRRH FDSNFKGGFN FYAGLKGYVL VLRTTSTVYN
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against DAO. It has been selected for its ability to recognize DAO in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: DAO (MBS2002759)
Biotin Conjugated Antibody: DAO (MBS2005277)
Matching Pairs
Unconjugated Antibody: DAO (MBS2002759)
Immunogen: DAO (MBS2011947)
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Molecular Weight
85,424 Da
3D Structure
ModBase 3D Structure for Q8JZQ5
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant DAO (His292~Asn460) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2045567
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-DAO antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-DAO antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-DAO antibody

Western Blot: Sample: Mouse Placenta lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse DAO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-DAO Polyclonal Antibody – Cat# MBS2002759

Western Blot (WB) of anti-DAO antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-DAO Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002759

Immunohistochemistry (IHC) of anti-DAO antibody

DAB staining on IHC-P. Samples: Mouse Tissue)
Rabbit Anti-DAO Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002759

Other Notes

Small volumes of anti-DAO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DAO. It may not necessarily be applicable to this product.

NCBI GI #
47605364
NCBI GeneID
76507
NCBI Accession #
Q8JZQ5.1
UniProt Primary Accession #
Q8JZQ5
UniProt Secondary Accession #
Q8R229; Q8VC36
UniProt Related Accession #
Q8JZQ5
NCBI Official Full Name
Amiloride-sensitive amine oxidase
NCBI Official Synonym Full Names
amine oxidase, copper-containing 1
NCBI Official Symbol
Aoc1
NCBI Official Synonym Symbols
DAO; Abp1; 1600012D06Rik
UniProt Protein Name
Amiloride-sensitive amine oxidase [copper-containing]
UniProt Synonym Protein Names
Amiloride-binding protein 1; Amine oxidase copper domain-containing protein 1; Histaminase
Protein Family
2-oxoglutarate-dependent dioxygenase
UniProt Gene Name
Aoc1
UniProt Synonym Gene Names
Abp1; DAO; Diamine oxidase
NCBI Protein Information
amiloride-sensitive amine oxidase [copper-containing]

UniProt Comments for DAO

ABP1: Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. Belongs to the copper/topaquinone oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - histidine; Amino Acid Metabolism - tryptophan; EC 1.4.3.22; Oxidoreductase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6 B2.3|6 23.78 cM

Cellular Component: bicellular tight junction; extracellular region; extracellular space; plasma membrane

Molecular Function: amine oxidase activity; calcium ion binding; copper ion binding; drug binding; heparin binding; metal ion binding; oxidoreductase activity; protein complex binding; protein homodimerization activity; quinone binding; receptor activity; zinc ion binding

Biological Process: amiloride transport; amine metabolic process; response to antibiotic; response to drug
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Research Articles on DAO

1. findings suggest that WT1-dependent control of polyamine breakdown, which is mediated by changes in AOC1 expression, has a role in kidney organogenesis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-DAO antibody

 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Histidine Metabolism Pathway antibodies  Histidine Metabolism Pathway Diagram
 Histidine Metabolism Pathway antibodies  Histidine Metabolism Pathway Diagram
 Tryptophan Metabolism Pathway antibodies  Tryptophan Metabolism Pathway Diagram
 Tryptophan Metabolism Pathway antibodies  Tryptophan Metabolism Pathway Diagram
 Tryptophan Metabolism Pathway antibodies  Tryptophan Metabolism Pathway Diagram
 Beta-alanine Biosynthesis I Pathway antibodies  Beta-alanine Biosynthesis I Pathway Diagram
 Histamine Degradation Pathway antibodies  Histamine Degradation Pathway Diagram

Organs/Tissues associated with anti-DAO antibody

 Organ/Tissue Name  Pubmed Publications
 Leaf Antibodies  >12 publications with DAO and Leaf
 Flower Antibodies  >4 publications with DAO and Flower
 Whole Plant Antibodies  >1 publications with DAO and Whole Plant
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