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Surfactant Associated Protein B (SPB) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002765
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MBS2002765 is an antibody targeting SPB. Also known as Biotin-Linked Antibody to Surfactant Associated Protein B (SPB). Reactive in Mouse. Host: Rabbit. More details

$220 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Surfactant Associated Protein B (SPB), Polyclonal Antibody
Full Product Name
Biotin-Linked Antibody to Surfactant Associated Protein B (SPB)
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/ml (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against SPB conjugated to biotin. It has been selected for its ability to recognize SPB in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Biotin Conjugated Antibody: SPB (MBS2002765)
Immunogen: SPB (MBS2012244)
Matching Pairs
Unconjugated Antibody: SPB (MBS2003121)
Biotin Conjugated Antibody: SPB (MBS2002765)
Molecular Weight
41,590 Da
Chromosome Location
Chromosome: 4; NC_005103.3 (164937638..164946703). Location: 4q33
3D Structure
ModBase 3D Structure for P22355
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Product Categories/Family for anti-SPB antibody

ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-SPB antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-SPB antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Other Notes

Small volumes of anti-SPB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
surfactant associated protein B
NCBI Official Synonym Full Names
surfactant protein B
NCBI Official Symbol
Sftpb
NCBI Official Synonym Symbols
Sp-b
UniProt Protein Name
Pulmonary surfactant-associated protein B
UniProt Synonym Protein Names
Pulmonary surfactant-associated proteolipid SPL(Phe)
UniProt Gene Name
Sftpb
UniProt Synonym Gene Names
Sftp3; SP-B
UniProt Entry Name
PSPB_RAT
NCBI Protein Information
pulmonary surfactant-associated protein B; surfactant associated protein B; surfactant pulmonary-associated protein B; surfactant, pulmonary-associated protein B; pulmonary surfactant-associated proteolipid SPL(Phe)

NCBI Summary for SPB

acts to lower surface tension in lung alveoli [RGD, Feb 2006]

UniProt Comments for SPB

Function: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

Subunit structure: Homodimer; disulfide-linked.

Subcellular location: Secreted › extracellular space › surface film.

Miscellaneous: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Sequence similarities: Contains 1 saposin A-type domain.Contains 3 saposin B-type domains.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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