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HEXA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200278

HEXA (Hexosaminidase A), also designated beta-Hexosaminidase A, is responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. A mutation in the a subunit of… More details

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Product Overview

Online Datasheet in PDF
Product Name
HEXA, Monoclonal Antibody
Full Product Name
HEXA antibody
Product Gene Name
Product Synonym Names
beta-hexosaminidase subunit alpha; TSD; hexosaminidase A
Clonality
Monoclonal
Isotype
IgG2a, l
Clone Number
AT20F1
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
19,326 Da
Antigen Species
Human
Immunogen
Recombinant human HEXA (89-529aa) purified from E. coli
Clone Note
Anti-human HEXA mAb, clone AT20F1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HEXA protein.

Product Categories/Family for anti-HEXA antibody

Applications Tested/Suitable for anti-HEXA antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-HEXA antibody
Recommended starting dilution is 1:3000.

Western Blot (WB) of anti-HEXA antibody

The cell lysates of MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HEXA antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HEXA antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HEXA Transfected 293T cell lysate
Mouse Anti-HEXA Monoclonal Antibody – Cat# MBS200278

Product References and Citations for anti-HEXA antibody

Cabezas. J.A. (1989) Biochem J 261 (3): 1059-1060; Lemieux. M.J., et al. (2006) J Mol Biol 359 (4): 913-929; Boles. D.J. and Proia. R.L. (1995) Am J Hum Genet 56 (3): 716-724

Other Notes

Small volumes of anti-HEXA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HEXA purchase

MBS200278 is an antibody targeting HEXA. Also known as HEXA antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HEXA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
hexosaminidase A preproprotein
NCBI Official Synonym Full Names
hexosaminidase subunit alpha
NCBI Official Symbol
HEXA
NCBI Official Synonym Symbols
TSD
UniProt Protein Name
Beta-hexosaminidase subunit alpha
UniProt Synonym Protein Names
Beta-N-acetylhexosaminidase subunit alpha; Hexosaminidase subunit A; N-acetyl-beta-glucosaminidase subunit alpha
Protein Family
UniProt Gene Name
HEXA
UniProt Synonym Gene Names
Hexosaminidase subunit A
NCBI Protein Information
beta-hexosaminidase subunit alpha

NCBI Summary for HEXA

This gene encodes a member of the glycosyl hydrolase 20 family of proteins. The encoded preproprotein is proteolytically processed to generate the alpha subunit of the lysosomal enzyme beta-hexosaminidase. This enzyme, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene lead to an accumulation of GM2 ganglioside in neurons, the underlying cause of neurodegenerative disorders termed the GM2 gangliosidoses, including Tay-Sachs disease (GM2-gangliosidosis type I). Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
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UniProt Comments for HEXA

Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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