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Transcription Factor A, Mitochondrial (TFAM) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002803
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MBS2002803 is an antibody targeting TFAM. Also known as Polyclonal Antibody to Transcription Factor A, Mitochondrial (TFAM). Reactive in Rat, Human, Mouse. Host: Rabbit. More details

$220 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Transcription Factor A, Mitochondrial (TFAM), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Transcription Factor A, Mitochondrial (TFAM)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-S LGNYPK KPMSSYLRFS TEQLPKFKAK HPDAKVSELI RKIAAMWREL PEAEKKVYEA DFKAEWKVYK EAVSKYKEQL TPSQLMGLEK EARQKRLKKK AQIKRRELIL LGKPKRPRSA YNIYVSESFQ EAKDESAQGK LKLVNQAWKN LSHDEKQAYI QLAKDDRIRY DNEMKSWEEQ MAEVGRSDLI RRSVKRPPGD ISEN
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat, Human, Mouse
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against TFAM. It has been selected for its ability to recognize TFAM in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: TFAM (MBS2002803)
Biotin Conjugated Antibody: TFAM (MBS2005852)
Matching Pairs
Unconjugated Antibody: TFAM (MBS2002803)
Immunogen: TFAM (MBS2011433)
Molecular Weight
– Da
3D Structure
ModBase 3D Structure for Q91ZW1
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant TFAM (Ser43-Asn244) expressed in E.coli.
Cross Reactivity
Human, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2079154
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-TFAM antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-TFAM antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-TFAM antibody

Western Blot: Sample: Rat Testis lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat TFAM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-TFAM Polyclonal Antibody – Cat# MBS2002803

Western Blot (WB) of anti-TFAM antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-TFAM Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002803

Immunohistochemistry (IHC) of anti-TFAM antibody

DAB staining on IHC-P; Samples: Rat Cardiac Muscle Tissue)
Rabbit Anti-TFAM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002803

Other Notes

Small volumes of anti-TFAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TFAM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor A, mitochondrial
NCBI Official Synonym Full Names
transcription factor A, mitochondrial
NCBI Official Symbol
Tfam
NCBI Official Synonym Symbols
Mttfa
UniProt Protein Name
Transcription factor A, mitochondrial
Protein Family
UniProt Gene Name
Tfam
UniProt Synonym Gene Names
mtTFA
NCBI Protein Information
transcription factor A, mitochondrial

NCBI Summary for TFAM

regulates transcription and replication of mitochondrial DNA [RGD, Feb 2006]

UniProt Comments for TFAM

Isoform Mitochondrial binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA (). Isoform Nuclear may also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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