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NBL1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200283

NBL1 is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and… More details

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Product Overview

Online Datasheet in PDF
Product Name
NBL1, Monoclonal Antibody
Full Product Name
NBL1 antibody
Product Gene Name
anti-NBL1 antibody
Product Synonym Names
Neuroblastoma suppressor of tumorigenicity 1; D1S1733E; DAN; DAND1; NB; NO3
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT38G8
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
OMIM
D28124 mRNA
3D Structure
ModBase 3D Structure for P41271
Antigen Species
Human
Immunogen
Recombinant human NBL1 (18-181aa) purified from E. coli
Clone Note
Anti-human NBL1 mAb, clone AT38G8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NBL1 protein.

Product Categories/Family for anti-NBL1 antibody

Cancer
Applications Tested/Suitable for anti-NBL1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-NBL1 antibody
Recommended starting dilution is 1:1000.

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Western Blot (WB) of anti-NBL1 antibody

The cell lysate of Jurkat (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-NBL1 Monoclonal Antibody – Cat# MBS200283

Immunofluorescence (IF) of anti-NBL1 antibody

CC/IF analysis of NBL1 in Jurkat cells. The cell was stained with MBS200283 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-NBL1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200283

Immunofluorescence (IF) of anti-NBL1 antibody

ICC/IF analysis of NBL1 in A549 cells. The cell was stained with MBS200283 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-NBL1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200283

Flow Cytometry (FC) of anti-NBL1 antibody

Flow cytometry analysis of NBL1 in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Mouse Anti-NBL1 Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS200283

Flow Cytometry (FC) of anti-NBL1 antibody

Flow cytometry analysis of NBL1 in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Mouse Anti-NBL1 Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS200283

Product References and Citations for anti-NBL1 antibody

Olakowski, M., et al. (2009) Folia Histochem Cytobiol 47 (2): 249-55; Shaikhibrahim, Z., et al. (2011) Int J Mol Med 28 (4): 605-11

Other Notes

Small volumes of anti-NBL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NBL1 purchase

MBS200283 is an antibody targeting NBL1. Also known as NBL1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NBL1. It may not necessarily be applicable to this product.

NCBI GI #
323276673
NCBI GeneID
4681
NCBI Accession #
NP_005371
UniProt Primary Accession #
P41271
UniProt Secondary Accession #
Q5TGZ2; Q5U0N4; Q96L68; A3KFI7
UniProt Related Accession #
P41271
GenBank Nucleotide #
NM_005380.7
NCBI Official Full Name
neuroblastoma suppressor of tumorigenicity 1 isoform 2
NCBI Official Synonym Full Names
neuroblastoma 1, DAN family BMP antagonist
NCBI Official Symbol
NBL1
NCBI Official Synonym Symbols
NB; DAN; NO3; DAND1; D1S1733E
UniProt Protein Name
Neuroblastoma suppressor of tumorigenicity 1
UniProt Synonym Protein Names
DAN domain family member 1; Protein N03; Zinc finger protein DAN
Protein Family
N-terminal-borealin-like protein
UniProt Gene Name
NBL1
UniProt Synonym Gene Names
DAN; DAND1
NCBI Protein Information
neuroblastoma suppressor of tumorigenicity 1

NCBI Summary for NBL1

This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]
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UniProt Comments for NBL1

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

Research Articles on NBL1

1. The structure of Grem2-GDF5 complex has revealed a number of key findings for DAN-family mediated BMP2 inhibition.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NBL1 antibody

 Products by Pathway  Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram

Diseases associated with anti-NBL1 antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >4 publications with NBL1 and Death
 Hypertension Antibodies  >3 publications with NBL1 and Hypertension
 Chromosome Aberrations Antibodies  >3 publications with NBL1 and Chromosome Aberrations
 Lung Neoplasms Antibodies  >2 publications with NBL1 and Lung Neoplasms
 Poisoning Antibodies  >1 publications with NBL1 and Poisoning
 Peripheral Nervous System Diseases Antibodies  >1 publications with NBL1 and Peripheral Nervous System Diseases
 Immune System Diseases Antibodies  >1 publications with NBL1 and Immune System Diseases

Organs/Tissues associated with anti-NBL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >26 publications with NBL1 and Bone
 Blood Antibodies  >8 publications with NBL1 and Blood
 Embryonic Tissue Antibodies  >6 publications with NBL1 and Embryonic Tissue
 Lung Antibodies  >5 publications with NBL1 and Lung
 Brain Antibodies  >5 publications with NBL1 and Brain
 Muscle Antibodies  >4 publications with NBL1 and Muscle
 Prostate Antibodies  >4 publications with NBL1 and Prostate
 Eye Antibodies  >3 publications with NBL1 and Eye
 Vascular Antibodies  >3 publications with NBL1 and Vascular
 Connective Tissue Antibodies  >3 publications with NBL1 and Connective Tissue
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