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Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002862
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MBS2002862 is an antibody targeting SIGLEC5. Also known as Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5). Reactive in Human. Host: Rabbit. More details

$205 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MHHHHHHSSG VPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-DIHF LEPLESGRPT RLSCSLPGSC EAGPPLTFSW TGNALSPLDP ETTRSSELTL TPRPEDHGTN LTCQMKRQGA QVTTERTVQL NVSYAPQTIT IFRNGIALEI LQNTSYLPVL EGQALRLLCD APSNPPAHLS WFQGSPALNA TPISNTGILE LRRVRSAEEG GFTCRAQHPL GFLQIFLNLS VYSLP
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against SIGLEC5. It has been selected for its ability to recognize SIGLEC5 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: SIGLEC5 (MBS2002862)
Immunogen: SIGLEC5 (MBS2010667)
Matching Pairs
Unconjugated Antibody: SIGLEC5 (MBS2002862)
APC-CY7 Conjugated Antibody: SIGLEC5 (MBS2050031)
Molecular Weight
60,715 Da
OMIM
AF170484 mRNA
3D Structure
ModBase 3D Structure for O15389
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant SIGLEC5 (Asp147~Pro335) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2050037
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-SIGLEC5 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-SIGLEC5 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-SIGLEC5 antibody

Western Blot: Sample: Human Serum; Primary Ab: 1ug/ml Rabbit Anti-Human SIGLEC5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-SIGLEC5 Polyclonal Antibody – Cat# MBS2002862

Western Blot (WB) of anti-SIGLEC5 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-SIGLEC5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002862

Immunohistochemistry (IHC) of anti-SIGLEC5 antibody

DAB staining on fromalin fixed paraffin-embedded Spleen tissue)
Rabbit Anti-SIGLEC5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002862

Other Notes

Small volumes of anti-SIGLEC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SIGLEC5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sialic acid-binding Ig-like lectin 5
NCBI Official Synonym Full Names
sialic acid binding Ig like lectin 5
NCBI Official Symbol
SIGLEC5
NCBI Official Synonym Symbols
CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
UniProt Protein Name
Sialic acid-binding Ig-like lectin 5
UniProt Synonym Protein Names
CD33 antigen-like 2; Obesity-binding protein 2; OB-BP2; OB-binding protein 2; CD_antigen: CD170
UniProt Gene Name
SIGLEC5
UniProt Synonym Gene Names
CD33L2; OBBP2; Siglec-5; OB-BP2; OB-binding protein 2
NCBI Protein Information
sialic acid-binding Ig-like lectin 5

NCBI Summary for SIGLEC5

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]
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UniProt Comments for SIGLEC5

SIGLEC5: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: integral component of membrane; plasma membrane; secretory granule membrane

Molecular Function: carbohydrate binding; protein binding

Biological Process: cell adhesion; neutrophil degranulation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SIGLEC5 antibody

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