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BMP8b Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200288

Bone morphogenic proteins (BMPs) are members of the TGF beta superfamily. BMPs are involved in the induction of cartilage and bone formation. Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial… More details

$285 / 0.05 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
BMP8b, Monoclonal Antibody
Full Product Name
BMP8b antibody
Product Gene Name
Product Synonym Names
Bone morphogenetic protein 8B; BMP8; OP2
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT13E6
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
39,151 Da
OMIM
M97016 mRNA
3D Structure
ModBase 3D Structure for P34820
Antigen Species
Human
Immunogen
Recombinant human BMP8b (264-402aa) purified from E. coli
Clone Note
Anti-human BMP8b mAb, clone AT13E6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BMP8b protein.

Product Categories/Family for anti-BMP8b antibody

Applications Tested/Suitable for anti-BMP8b antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-BMP8b antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-BMP8b Monoclonal Antibody – Cat# MBS200288

Immunofluorescence (IF) of anti-BMP8b antibody

ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with ATGA0332 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-BMP8b Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200288

Product References and Citations for anti-BMP8b antibody

Ozkaynak E., et al. (1992) J Biol Chem. 267 (35):25220-25227; Gregory SG., et al. (2006) Nature. 441 (7091):315-321.

Other Notes

Small volumes of anti-BMP8b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BMP8b purchase

MBS200288 is an antibody targeting BMP8b. Also known as BMP8b antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BMP8b. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
bone morphogenetic protein 8B preproprotein
NCBI Official Synonym Full Names
bone morphogenetic protein 8b
NCBI Official Symbol
BMP8B
NCBI Official Synonym Symbols
OP2; BMP8
UniProt Protein Name
Bone morphogenetic protein 8B
UniProt Synonym Protein Names
Osteogenic protein 2; OP-2
UniProt Gene Name
BMP8B
UniProt Synonym Gene Names
BMP8; BMP-8; BMP-8B; OP-2
NCBI Protein Information
bone morphogenetic protein 8B

NCBI Summary for BMP8b

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The encoded protein stimulates thermogenesis in brown adipose tissue. Expression of this gene may be downregulated in pancreatic cancer. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 1. [provided by RefSeq, Jul 2016]
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UniProt Comments for BMP8b

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis ().

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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