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Semaphorin 5b (SEMA5B) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002880
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MBS2002880 is an antibody targeting SEMA5B. Also known as Polyclonal Antibody to Semaphorin 5B (SEMA5B). Reactive in Human, Rat. Host: Rabbit. More details

$225 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
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Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Semaphorin 5B (SEMA5B), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Semaphorin 5B (SEMA5B)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF- LLSLV RGLLPCLPPG ARTAEGPIMV LAGPLAVSLL LPSLTLLVSH LSSSQDVSSE PSSEQQLCAL SKHPTVAFED LQPWVSNFTY PGARDFSQLA LDPSGNQLIV GARNYLFRLS LANVSLLQAT E
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against SEMA5B. It has been selected for its ability to recognize SEMA5B in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: SEMA5B (MBS2002880)
Biotin Conjugated Antibody: SEMA5B (MBS2001874)
Matching Pairs
Unconjugated Antibody: SEMA5B (MBS2002880)
Immunogen: SEMA5B (MBS2011011)
Molecular Weight
131,904 Da
OMIM
AB040878 mRNA
3D Structure
ModBase 3D Structure for Q9P283
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant SEMA5B (Leu36~Glu161) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083422
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-SEMA5B antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-SEMA5B antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-SEMA5B antibody

Western Blot: Sample: Recombinant SEMA5B, Human.
Rabbit Anti-SEMA5B Polyclonal Antibody – Cat# MBS2002880

Immunohistochemistry (IHC) of anti-SEMA5B antibody

DABstainingonIHC-P.Samples:HumanTissue)
Rabbit Anti-SEMA5B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002880

Other Notes

Small volumes of anti-SEMA5B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SEMA5B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
semaphorin-5B isoform 1
NCBI Official Synonym Full Names
semaphorin 5B
NCBI Official Symbol
SEMA5B
NCBI Official Synonym Symbols
SemG; SEMAG
UniProt Protein Name
Semaphorin-5B
Protein Family
UniProt Gene Name
SEMA5B
UniProt Synonym Gene Names
KIAA1445; SEMAG
NCBI Protein Information
semaphorin-5B

NCBI Summary for SEMA5B

This gene encodes a member of the semaphorin protein family which regulates axon growth during development of the nervous system. The encoded protein has a characteristic Sema domain near the N-terminus, through which semaphorins bind to plexin, and five thrombospondin type 1 repeats in the C-terminal region of the protein. The protein product may be cleaved and exist as a secreted molecule (PMID: 19463192). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
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UniProt Comments for SEMA5B

SEMA5B: a single-pass type I membrane protein of the semaphorin family that may act as positive axonal guidance cues. Two alternatively spliced human isoforms have been described.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21.1

Cellular Component: extracellular space; integral component of membrane

Biological Process: axon extension; detection of light stimulus involved in visual perception; negative chemotaxis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SEMA5B antibody

Diseases associated with anti-SEMA5B antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with SEMA5B and Neoplasms
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