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Beta-1,3-N-Acetylglucosaminyltransferase Manic Fringe (MFNG) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002918
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MBS2002918 is an antibody targeting MFNG. Also known as Polyclonal Antibody to Beta-1,3-N-Acetylglucosaminyltransferase Manic Fringe (MFNG). Reactive in Human. Host: Rabbit. More details

$225 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Beta-1,3-N-Acetylglucosaminyltransferase Manic Fringe (MFNG), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Beta-1,3-N-Acetylglucosaminyltransferase Manic Fringe (MFNG)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-S RTREQTFVFT DSPDKGLQER LGSHLVVTNC SAEHSHPALS CKMAAEFDTF LASGLRWFCH VDDDNYVNPR ALLQLLRAFP LARDVYVGRP SLNRPIHASE PQPHNRTRLV QFWFATGGAG FCINRKLALK MAPWASGSRF MDTSALIRLP DDCTMGYIIE CKLGGRLQPS PLFHSHLETL QLLRTAQLPE QVTLSYGVFE GKLNVIKLQG PFSPEEDPSR FRSLHCLLYP DTPWCP
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against MFNG. It has been selected for its ability to recognize MFNG in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: MFNG (MBS2002918)
Immunogen: MFNG (MBS2011608)
Matching Pairs
Unconjugated Antibody: MFNG (MBS2002918)
APC-CY7 Conjugated Antibody: MFNG (MBS2083195)
Molecular Weight
34,666 Da
OMIM
U94352 mRNA
3D Structure
ModBase 3D Structure for O00587
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant MFNG (Ser80~Pro316) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083197
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-MFNG antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-MFNG antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-MFNG antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-MFNG Polyclonal Antibody – Cat# MBS2002918

Immunohistochemistry (IHC) of anti-MFNG antibody

DAB staining on IHC-P; Samples: Human Liver Tissue)
Rabbit Anti-MFNG Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002918

Other Notes

Small volumes of anti-MFNG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MFNG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform 2
NCBI Official Synonym Full Names
MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
NCBI Official Symbol
MFNG
UniProt Protein Name
Beta-1,3-N-acetylglucosaminyltransferase manic fringe
UniProt Synonym Protein Names
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
UniProt Gene Name
MFNG
NCBI Protein Information
beta-1,3-N-acetylglucosaminyltransferase manic fringe

NCBI Summary for MFNG

This gene is a member of the fringe gene family which also includes radical and lunatic fringe genes. They all encode evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. [provided by RefSeq, Oct 2009]
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UniProt Comments for MFNG

MFNG: Glycosyltransferase involved in the elongation of O- linked ligands to activate Notch signaling. Possesses fucose- specific beta-1,3-N-acetylglucosaminyltransferase activity. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.1.222; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular space; integral to Golgi membrane

Molecular Function: metal ion binding; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity

Biological Process: marginal zone B cell differentiation; pattern specification process; positive regulation of Notch signaling pathway; positive regulation of protein binding; regulation of Notch signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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