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ING2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200295

ING2 is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis. This protein… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ING2, Monoclonal Antibody
Full Product Name
ING2 antibody
Product Gene Name
Product Synonym Names
inhibitor of growth protein 2; ING1L; P33ing2
Clonality
Monoclonal
Isotype
IgG1b,k
Clone Number
AT39E5
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
27,984 Da
OMIM
AB012853 mRNA
3D Structure
ModBase 3D Structure for Q9H160
Antigen Species
Human
Immunogen
Recombinant human ING2 (1-280aa) purified from E. coli
Clone Note
Anti-human ING2 mAb, clone AT39E5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ING2 protein.

Product Categories/Family for anti-ING2 antibody

Applications Tested/Suitable for anti-ING2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ING2 antibody
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-ING2 antibody

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ING2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-ING2 Monoclonal Antibody – Cat# MBS200295

Immunofluorescence (IF) of anti-ING2 antibody

ICC/IF analysis of ING2 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ING2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-ING2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200295

Product References and Citations for anti-ING2 antibody

Entrez Gene: ING2 inhibitor of growth family, member 2; Shimada Y., et al. (1999) Cytogenet Cell Genet 83 (3-4): 232-5; Nagashima M., et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98: 9671-9676; Gozani O., et al. (2003) Cell 114: 99-111;

Other Notes

Small volumes of anti-ING2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ING2 purchase

MBS200295 is an antibody targeting ING2. Also known as ING2 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ING2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
inhibitor of growth protein 2 isoform 1
NCBI Official Synonym Full Names
inhibitor of growth family member 2
NCBI Official Symbol
ING2
NCBI Official Synonym Symbols
ING1L; p33ING2
UniProt Protein Name
Inhibitor of growth protein 2
UniProt Synonym Protein Names
Inhibitor of growth 1-like protein; ING1Lp; p32; p33ING2
UniProt Gene Name
ING2
UniProt Synonym Gene Names
ING1L; ING1Lp
NCBI Protein Information
inhibitor of growth protein 2

NCBI Summary for ING2

This gene is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
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UniProt Comments for ING2

Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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