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CACYBP Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200296

CACYBP (Calcyclin-binding protein) is primarily a nuclear protein that contains one CS domain and one SGS domain. CACYBP is believed to be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It most likely… More details

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Product Overview

Online Datasheet in PDF
Product Name
CACYBP, Monoclonal Antibody
Full Product Name
CACYBP antibody
Product Gene Name
Product Synonym Names
Clcyclin-binding protein; GIG5; PNAS-107; RP1-102G20.6; S100A6BP; SIP; Clcyclin-binding protein CACYBP; Calcyclin binding protein; GIG 5; Growth inhibiting gene 5; Growth inhibiting gene 5 protein; hCacyBP; MGC87971; PNAS 107; PNAS107; RP1 102G20.6; S100A6 binding protein.
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT3G8
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
21,228 Da
OMIM
AF057356 mRNA
3D Structure
ModBase 3D Structure for Q9HB71
Antigen Species
Human
Immunogen
Recombinant human CACYBP (1-185aa) purified from E. coli
Clone Note
Anti-human CACYBP mAb, clone AT3G8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CACYBP protein

Product Categories/Family for anti-CACYBP antibody

Applications Tested/Suitable for anti-CACYBP antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-CACYBP antibody
Recommended starting dilution is 1:1000

Western Blot (WB) of anti-CACYBP antibody

The cell lysates of HeLa, 293T, Jurkat and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CACYBP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-CACYBP Monoclonal Antibody – Cat# MBS200296

Product References and Citations for anti-CACYBP antibody

Ghosh. D., et al. (2013) Mol Cell Proteomics 12 (7): 1865-1880; Rines. A.K., et al. (2012) FASEB 26 (11): 4685-4695; Ning. X., et al. (2007) Mol Cancer Res 5 (12): 1254-1262

Other Notes

Small volumes of anti-CACYBP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CACYBP purchase

MBS200296 is an antibody targeting CACYBP. Also known as CACYBP antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CACYBP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
calcyclin-binding protein isoform 2
NCBI Official Synonym Full Names
calcyclin binding protein
NCBI Official Symbol
CACYBP
NCBI Official Synonym Symbols
SIP; GIG5; PNAS-107; S100A6BP
UniProt Protein Name
Calcyclin-binding protein
UniProt Synonym Protein Names
S100A6-binding protein; Siah-interacting protein
UniProt Gene Name
CACYBP
UniProt Synonym Gene Names
S100A6BP; SIP; CacyBP; hCacyBP
NCBI Protein Information
calcyclin-binding protein

NCBI Summary for CACYBP

The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for CACYBP

May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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