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CD46 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200297

Membrane Cofactor Protein (CD46) is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 regulates the complement cascade by inhibiting C3b and C4b deposited on self tissue. This protein is a cofactor that binds to… More details

$285 / 0.05 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CD46, Monoclonal Antibody
Full Product Name
CD46 antibody
Product Gene Name
Product Synonym Names
Membrane cofactor protein isoform 14; AHuS2; MCP; MIC10; TLX; TRA2.10
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT2G9
Host
Mouse
Species Reactivity
Human
Form/Format
In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
For long term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing cycles.
OMIM
Y00651 mRNA
3D Structure
ModBase 3D Structure for P15529
Immunogen
Recombinant human CD46 (35-313aa) purified from E. coli

Product Categories/Family for anti-CD46 antibody

Applications Tested/Suitable for anti-CD46 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-CD46 antibody
The antibody has been tested by ELISA, Western blot, ICC/IF, FC and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Western Blot (WB) of anti-CD46 antibody

The cell lysates of Ramos, HeLa and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD46 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-CD46 Monoclonal Antibody – Cat# MBS200297

Immunofluorescence (IF) of anti-CD46 antibody

ICC/IF analysis of CD46 in HeLa cells. The cell was stained with MBS200297 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-CD46 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200297

Flow Cytometry (FC) of anti-CD46 antibody

Flow cytometry analysis of CD46 in PBMC cells. The cell was stained with ATGA0326 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Mouse Anti-CD46 Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS200297

Immunohistochemistry (IHC) of anti-CD46 antibody

Paraffin embedded sections of human colorectal cancer tissue were incubated with anti-human CD46 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine(DAB).
Mouse Anti-CD46 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200297

Product References and Citations for anti-CD46 antibody

Liszewski M.K., et al. (1991) Annu Rev Immunol 9: 431-455; Riley-Varqas R.C., et al. (2004) Trends Immunol 25 (9): 496-503; Studebaker A.W., et al. (2010) Neuro Oncol 12: 1034-1042

Other Notes

Small volumes of anti-CD46 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD46 purchase

MBS200297 is an antibody targeting CD46. Also known as CD46 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD46. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
membrane cofactor protein isoform 14
NCBI Official Synonym Full Names
CD46 molecule
NCBI Official Symbol
CD46
NCBI Official Synonym Symbols
MCP; TLX; AHUS2; MIC10; TRA2.10
UniProt Protein Name
Membrane cofactor protein
UniProt Synonym Protein Names
TLX; Trophoblast leukocyte common antigen; CD_antigen: CD46
UniProt Gene Name
CD46
UniProt Synonym Gene Names
MCP; MIC10
NCBI Protein Information
membrane cofactor protein

NCBI Summary for CD46

The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]
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UniProt Comments for CD46

Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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