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5'-Nucleotidase, Cytosolic III (NT5C3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2003007
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MBS2003007 is an antibody targeting NT5C3. Also known as Polyclonal Antibody to 5'-Nucleotidase, Cytosolic III (NT5C3). Reactive in Mouse, Human, Rat. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
5'-Nucleotidase, Cytosolic III (NT5C3), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to 5'-Nucleotidase, Cytosolic III (NT5C3)
Product Gene Name
anti-NT5C3 antibody
Immunogen Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSG SEF-MTNQES AVHL KMMPEFQKSS VRIKNPTRVE EIICGLIKGG AAKLQIITDF DMTLSRFSYN GKRCPTCHNI IDNCKLVTDE CRRKLLQLKE QYYAIEVDPV LTVEEKFPYM VEWYTKSHGL LIEQGIPKAK LKEIVADSDV MLKEGYENFF GKLQQHGIPV FIFSAGIGDV LEEVIRQAGV YHSNVKVVSN FMDFDENGVL KGFKGELIHV FNKHDGALKN TDYFSQLKDN SNIILLGDSQ GDLRMADGVA NVEHILKIGY LNDRVDELLE KYMDSYDIVL VKEESLEVVN SILQKTL
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Human, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against NT5C3. It has been selected for its ability to recognize NT5C3 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: NT5C3 (MBS2003007)
Biotin Conjugated Antibody: NT5C3 (MBS2005861)
Matching Pairs
Unconjugated Antibody: NT5C3 (MBS2003007)
Immunogen: NT5C3 (MBS2011361)
Show more
Molecular Weight
33,790 Da
3D Structure
ModBase 3D Structure for Q9D020
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant NT5C3 (Met1~Leu297) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2073663
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-NT5C3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-NT5C3 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-NT5C3 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-NT5C3 Polyclonal Antibody – Cat# MBS2003007

Immunohistochemistry (IHC) of anti-NT5C3 antibody

DAB staining on fromalin fixed paraffin-embedded stomach tissue)
Rabbit Anti-NT5C3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2003007

Other Notes

Small volumes of anti-NT5C3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NT5C3. It may not necessarily be applicable to this product.

NCBI GI #
356991161
NCBI GeneID
107569
NCBI Accession #
NP_001239303.1
UniProt Primary Accession #
Q9D020
UniProt Secondary Accession #
Q8CI05; Q9D0D9
UniProt Related Accession #
Q9D020
GenBank Nucleotide #
NM_001252374.1
NCBI Official Full Name
cytosolic 5'-nucleotidase 3A isoform 2
NCBI Official Synonym Full Names
5'-nucleotidase, cytosolic III
NCBI Official Symbol
Nt5c3
NCBI Official Synonym Symbols
p36; Nt5y; PN-1; PN-I; PSN1; UMPH; Umph1; lupin; Nt5c3a; Umph-1; cN-III; AI842776; 1600024P05Rik; 2610206B05Rik; 3110004A18Rik
UniProt Protein Name
Cytosolic 5'-nucleotidase 3A
UniProt Synonym Protein Names
7-methylguanosine phosphate-specific 5'-nucleotidase
Protein Family
Cytosolic 5'-nucleotidase
UniProt Gene Name
Nt5c3a
UniProt Synonym Gene Names
Nt5c3; 7-methylguanosine nucleotidase; cN-III; P5'N-1; P5N-1; PN-I
NCBI Protein Information
cytosolic 5'-nucleotidase 3A

UniProt Comments for NT5C3

NT5C3A: Can act both as nucleotidase and as phosphotransferase. Defects in NT5C3 are the cause of P5N deficiency (P5ND); also called hemolytic anemia due to P5N deficiency or hemolytic anemia due to UMPH1 deficiency. P5ND is an autosomal recessive condition causing hemolytic anemia characterized by marked basophilic stipplig and the accumulation of high concentrations of pyrimidine nucleotides within the erythrocyte. It is implicated in the anemia of lead poisoning and is possibly associated with learning difficulties. Belongs to the pyrimidine 5'-nucleotidase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.5; Endoplasmic reticulum; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein); Transferase

Chromosomal Location of Human Ortholog: 6|6 B3

Cellular Component: cytoplasm; endoplasmic reticulum; mitochondrion

Molecular Function: 5'-nucleotidase activity; hydrolase activity; magnesium ion binding; metal ion binding; nucleotide binding; transferase activity

Biological Process: adenosine metabolic process; defense response to virus; nucleotide metabolic process
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Research Articles on NT5C3

1. report describes the first X-ray structure of a catalytically inactivated variant of murine cN-III with a natural substrate, uridine 5'-monophosphate, in the active site at 1.74A resolution

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NT5C3 antibody

 Reference Product  PubMed Publications
 Dck antibody  >3 publications with NT5C3 and Dck
 Dctd antibody  >1 publications with NT5C3 and Dctd

Pathways associated with anti-NT5C3 antibody

 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Catabolism Pathway antibodies  Pyrimidine Catabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram

Diseases associated with anti-NT5C3 antibody

 Disease Name  Pubmed Publications
 Anemia Antibodies  >5 publications with NT5C3 and Anemia
 Disease Models, Animal Antibodies  >2 publications with NT5C3 and Disease Models, Animal
 Poisoning Antibodies  >1 publications with NT5C3 and Poisoning
 Nervous System Diseases Antibodies  >1 publications with NT5C3 and Nervous System Diseases
 Liver Diseases Antibodies  >1 publications with NT5C3 and Liver Diseases
 Vascular Diseases Antibodies  >1 publications with NT5C3 and Vascular Diseases
 Cardiovascular Diseases Antibodies  >1 publications with NT5C3 and Cardiovascular Diseases
 Fibrosis Antibodies  >1 publications with NT5C3 and Fibrosis
 Mental Disorders Antibodies  >1 publications with NT5C3 and Mental Disorders
 Kidney Diseases Antibodies  >1 publications with NT5C3 and Kidney Diseases

Organs/Tissues associated with anti-NT5C3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7 publications with NT5C3 and Blood
 Liver Antibodies  >5 publications with NT5C3 and Liver
 Stomach Antibodies  >2 publications with NT5C3 and Stomach
 Lung Antibodies  >1 publications with NT5C3 and Lung
 Kidney Antibodies  >1 publications with NT5C3 and Kidney
 Skin Antibodies  >1 publications with NT5C3 and Skin
 Heart Antibodies  >1 publications with NT5C3 and Heart
 Bone Marrow Antibodies  >1 publications with NT5C3 and Bone Marrow
 Spleen Antibodies  >1 publications with NT5C3 and Spleen
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