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PDCL3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200313

PDCL3 (phosducin-like 3), also known as VIAF1 (viral IAP-associated factor 1), VIAF, PHLP3 or HTPHLP, is a widely expressed protein that belongs to the phosducin-like family of proteins. phosducin-like family members associate with the cytosolic chaperonin… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PDCL3, Monoclonal Antibody
Full Product Name
PDCL3 antibody
Product Gene Name
Product Synonym Names
Phosducin-Like protein 3; Phosducin-Like protein 3; HTPHLP; MGC3062; PHLP2A; PHLP3; VIAF; VIAF1
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT8F9
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
27,614 Da
OMIM
AF110511 mRNA
3D Structure
ModBase 3D Structure for Q9H2J4
Antigen Species
Human
Immunogen
Recombinant human PDCL3 (1-239aa) purified from E. coli
Clone Note
Anti-human PDCL3 mAb, clone AT8F9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDCL3 protein.

Product Categories/Family for anti-PDCL3 antibody

Applications Tested/Suitable for anti-PDCL3 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-PDCL3 antibody
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-PDCL3 antibody

Fig. 1: The extract of Jurkat (40ug), A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCL3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Fig. 2: The Human Recombinant protein PDCL3 (100ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCL3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-PDCL3 Monoclonal Antibody – Cat# MBS200313

Immunofluorescence (IF) of anti-PDCL3 antibody

ICC/IF analysis of PDCL3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDCL3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of PDCL3 in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDCL3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-PDCL3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200313

Product References and Citations for anti-PDCL3 antibody

Wilkinson J.C., et al. (2004) Biol. Chem. 279 (49):51091-51099; Hillier L.W., et al. (2005) Nature 434 (7034):724-731

Other Notes

Small volumes of anti-PDCL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDCL3 purchase

MBS200313 is an antibody targeting PDCL3. Also known as PDCL3 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PDCL3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosducin-like protein 3
NCBI Official Synonym Full Names
phosducin like 3
NCBI Official Symbol
PDCL3
NCBI Official Synonym Symbols
VIAF; PHLP3; VIAF1; HTPHLP; PHLP2A
UniProt Protein Name
Phosducin-like protein 3
UniProt Synonym Protein Names
HTPHLP; PhPL3; Viral IAP-associated factor 1; VIAF-1
UniProt Gene Name
PDCL3
UniProt Synonym Gene Names
PhLP2A; VIAF1; VIAF-1
NCBI Protein Information
phosducin-like protein 3

NCBI Summary for PDCL3

This gene encodes a member of the phosducin-like protein family and is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin. Members of the phosducin-like protein family have been shown to bind to the beta-gamma subunits of G proteins. [provided by RefSeq, Jul 2008]
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UniProt Comments for PDCL3

Acts as a chaperone for the angiogenic VEGF receptor KDR/VEGFR2, controlling its abundance and inhibiting its ubiquitination and degradation. Modulates the activation of caspases during apoptosis. Is a substrate for Orgyia pseudotsugata multicapsid polyhedrosis virus (OpMNPV) IAP-mediated ubiquitination.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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