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Transmembrane Protein 27 (TMEM27) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2003169
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MBS2003169 is an antibody targeting TMEM27. Also known as Polyclonal Antibody to Transmembrane Protein 27 (TMEM27). Reactive in Human, Mouse. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Transmembrane Protein 27 (TMEM27), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Transmembrane Protein 27 (TMEM27)
Product Gene Name
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF- CQPG AENAFKVRLS IRTALGDKAY AWDTNEEYLF KAMVAFSMRK VPNREATEIS HVLLCNVTQR VSFWFVVTDP SKNHTLPAVE VQSAIRMNKN RINNAFFLND QTLEFLKIPS TLAPPMD
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against TMEM27. It has been selected for its ability to recognize TMEM27 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: TMEM27 (MBS2003169)
Biotin Conjugated Antibody: TMEM27 (MBS2002478)
Matching Pairs
Unconjugated Antibody: TMEM27 (MBS2003169)
Immunogen: TMEM27 (MBS2012132)
Molecular Weight
25,235 Da
OMIM
AF229179 mRNA
3D Structure
ModBase 3D Structure for Q9HBJ8
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant TMEM27 (Cys17~Asp137) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2082304
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-TMEM27 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-TMEM27 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-TMEM27 antibody

Western Blot: Sample: Mouse Kidney Tissue.
Rabbit Anti-TMEM27 Polyclonal Antibody – Cat# MBS2003169

Western Blot (WB) of anti-TMEM27 antibody

Western Blot: Sample: Recombinant TMEM27, Human.
Rabbit Anti-TMEM27 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2003169

Immunohistochemistry (IHC) of anti-TMEM27 antibody

DAB staining on IHC-P. Samples: Human Tissue)
Rabbit Anti-TMEM27 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2003169

Other Notes

Small volumes of anti-TMEM27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TMEM27. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
collectrin
NCBI Official Synonym Full Names
collectrin, amino acid transport regulator
NCBI Official Symbol
CLTRN
NCBI Official Synonym Symbols
NX17; NX-17; TMEM27
UniProt Protein Name
Collectrin
UniProt Synonym Protein Names
Transmembrane protein 27
Protein Family
UniProt Gene Name
TMEM27
NCBI Protein Information
collectrin

NCBI Summary for TMEM27

This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma membrane. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. The extracellular domain of the encoded protein may be cleaved and shed from the plasma membrane specifically in pancreatic beta cells. [provided by RefSeq, Jun 2013]
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UniProt Comments for TMEM27

TMEM27: Regulator of SNARE complex function. Stimulator of beta cell replication. Belongs to the TMEM27 family.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: brush border membrane; cytoplasm; integral component of membrane

Molecular Function: metallopeptidase activity; peptidyl-dipeptidase activity; protein binding

Biological Process: positive regulation of amino acid transport; positive regulation of calcium ion-dependent exocytosis; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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