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CSTF1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200326

CSTF1, also known as cleavage stimulation factor subunit 1, is 431 amino acid protein. It is involved in the polyadenylation and 3 end cleavage of pre-mRNAs. CSTF1 may be responsible for the interaction of CSTF with other factors to form a stable complex on… More details

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Product Overview

Product Name
CSTF1, Monoclonal Antibody
Full Product Name
CSTF1 antibody
Product Gene Name
anti-CSTF1 antibody
Product Synonym Names
Cleavage stimulation factor subunit 1; CstF-50; CstFp50
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
AT10E11
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
48,358 Da
OMIM
L02547 mRNA
3D Structure
ModBase 3D Structure for Q05048
Antigen Species
Human
Immunogen
Recombinant human CSTF1 (1-431aa) purified from E. coli
Clone Note
Anti-human CSTF1 mAb, clone AT10E11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CSTF1 protein.

Product Categories/Family for anti-CSTF1 antibody

Epigenomics
Applications Tested/Suitable for anti-CSTF1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-CSTF1 antibody
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-CSTF1 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: MCF7 cell lysate Lane 2.: 293T cell lysate Lane 3.: HeLa cell lysate Lane 4.: A549 cell lysate Lane 5.: K562 cell lysate Lane 6.: Jurkat cell lysate
Mouse Anti-CSTF1 Monoclonal Antibody – Cat# MBS200326

Immunofluorescence (IF) of anti-CSTF1 antibody

ICC/IF analysis of CSTF1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CSTF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-CSTF1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200326

Product References and Citations for anti-CSTF1 antibody

Takagaki. Y. and Manley J.L. (1992) J Biol Chem 267: 23471-23474; Takagaki. Y. and Manley J.L. (2000) Mol Cell Biol 20: 1515-1525; Kleiman. F.E. and Manley. J.L. (1999) Science 285: 1576-1579;

Other Notes

Small volumes of anti-CSTF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CSTF1 purchase

MBS200326 is an antibody targeting CSTF1. Also known as CSTF1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CSTF1. It may not necessarily be applicable to this product.

NCBI GI #
75709217
NCBI GeneID
1477
NCBI Accession #
NP_001028693
UniProt Primary Accession #
Q05048
UniProt Secondary Accession #
Q5QPD8
UniProt Related Accession #
Q05048
GenBank Nucleotide #
NM_001033521.1
NCBI Official Full Name
cleavage stimulation factor subunit 1
NCBI Official Synonym Full Names
cleavage stimulation factor subunit 1
NCBI Official Symbol
CSTF1
NCBI Official Synonym Symbols
CstF-50; CstFp50
UniProt Protein Name
Cleavage stimulation factor subunit 1
UniProt Synonym Protein Names
CF-1 50 kDa subunit; Cleavage stimulation factor 50 kDa subunit; CSTF 50 kDa subunit; CstF-50
Protein Family
Cleavage stimulation factor
UniProt Gene Name
CSTF1
UniProt Synonym Gene Names
CSTF 50 kDa subunit; CstF-50
NCBI Protein Information
cleavage stimulation factor subunit 1

NCBI Summary for CSTF1

This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for CSTF1

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.

Research Articles on CSTF1

1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CSTF1 antibody

 Reference Product  PubMed Publications
 BARD1 antibody  >1 publications with CSTF1 and BARD1

Pathways associated with anti-CSTF1 antibody

 Products by Pathway  Pathway Diagram
 BARD1 Signaling Events Pathway antibodies  BARD1 Signaling Events Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Processing Of Capped Intronless Pre-mRNA Pathway antibodies  Processing Of Capped Intronless Pre-mRNA Pathway Diagram
 Processing Of Intronless Pre-mRNAs Pathway antibodies  Processing Of Intronless Pre-mRNAs Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Termination Pathway antibodies  RNA Polymerase II Transcription Termination Pathway Diagram
 MRNA 3'-end Processing Pathway antibodies  MRNA 3'-end Processing Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram

Diseases associated with anti-CSTF1 antibody

 Disease Name  Pubmed Publications
 Neoplasms, Experimental Antibodies  >1 publications with CSTF1 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >1 publications with CSTF1 and Breast Neoplasms

Organs/Tissues associated with anti-CSTF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Mammary Gland Antibodies  >1 publications with CSTF1 and Mammary Gland
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