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YWHAG Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200332

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, Beta, Gamma,… More details

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Product Overview

Online Datasheet in PDF
Product Name
YWHAG, Monoclonal Antibody
Full Product Name
YWHAG antibody
Product Gene Name
anti-YWHAG antibody
Product Synonym Names
Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein gamma polypeptide; YWHAG; 14-3-3 gamma,Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; gamma polypeptide; Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein; gamma polypeptide 14 3 3 protein gamma; 14 3 3 protein gamma subtype; 14 3 3gamma; 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide; KCIP 1; KCIP1; Protein kinase C inhibitor protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide.
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT4B9
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
28,303 Da
OMIM
AF142498 mRNA
3D Structure
ModBase 3D Structure for P61981
Antigen Species
Human
Immunogen
Recombinant human 14-3-3 gamma (1-247aa) purified from E. coli
Clone Note
Anti-human 14-3-3 gamma mAb, clone AT4B9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human 14-3-3 gamma protein.

Product Categories/Family for anti-YWHAG antibody

Signal Transduction
Applications Tested/Suitable for anti-YWHAG antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-YWHAG antibody
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-YWHAG antibody

The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human YWHAZ Lane 2.: Recombinant Human YWHAB Lane 3.: Recombinant Human YWHAE Lane 4.: Recombinant Human YWHAH Lane 5.: Recombinant Human YWHAG Lane 6.: Recombinant Human SFN Lane 7.: Recombinant Human YWHAQ The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate
Mouse Anti-YWHAG Monoclonal Antibody – Cat# MBS200332

Immunofluorescence (IF) of anti-YWHAG antibody

ICC/IF analysis of 14-3-3 gamma in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 gamma antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-YWHAG Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200332

Product References and Citations for anti-YWHAG antibody

Takagaki Y. and Manley J.L. (1992) J Biol Chem 267: 23471-23474; Takagaki Y. and Manley J.L. (2000) Mol Cell Biol 20: 1515-1525; Kleiman F.E. and Manley J.L. (1999) Science 285: 1576-1579

Other Notes

Small volumes of anti-YWHAG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for YWHAG purchase

MBS200332 is an antibody targeting YWHAG. Also known as YWHAG antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for YWHAG. It may not necessarily be applicable to this product.

NCBI GI #
21464101
NCBI GeneID
7532
NCBI Accession #
NP_036611
UniProt Primary Accession #
P61981
UniProt Secondary Accession #
O70457; P35214; Q6FH52; Q9UDP2; Q9UN99
UniProt Related Accession #
P61981
GenBank Nucleotide #
NM_012479.3
NCBI Official Full Name
14-3-3 protein gamma
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
NCBI Official Symbol
YWHAG
NCBI Official Synonym Symbols
EIEE56; PPP1R170; 14-3-3GAMMA
UniProt Protein Name
14-3-3 protein gamma
UniProt Synonym Protein Names
Protein kinase C inhibitor protein 1; KCIP-1
Protein Family
14-3-3 protein
UniProt Gene Name
YWHAG
UniProt Synonym Gene Names
KCIP-1
NCBI Protein Information
14-3-3 protein gamma

NCBI Summary for YWHAG

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]
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UniProt Comments for YWHAG

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
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Research Articles on YWHAG

1. hypermethylation of the 14-3-3 gene promoter accounts for the decreased 14-3-3 gamma in uterine leiomyomas and that such a low level of expression may be involved in the pathogenesis of leiomyomas

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-YWHAG antibody

 Reference Product  PubMed Publications
 YWHAZ antibody  >3 publications with YWHAG and YWHAZ
 YWHAB antibody  >2 publications with YWHAG and YWHAB

Pathways associated with anti-YWHAG antibody

 Products by Pathway  Pathway Diagram
 AURKA Activation By TPX2 Pathway antibodies  AURKA Activation By TPX2 Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Anchoring Of The Basal Body To The Plasma Membrane Pathway antibodies  Anchoring Of The Basal Body To The Plasma Membrane Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram

Diseases associated with anti-YWHAG antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >8 publications with YWHAG and Nervous System Diseases
 Neurobehavioral Manifestations Antibodies  >5 publications with YWHAG and Neurobehavioral Manifestations
 Congenital Abnormalities Antibodies  >4 publications with YWHAG and Congenital Abnormalities
 Heart Diseases Antibodies  >4 publications with YWHAG and Heart Diseases
 Cardiovascular Diseases Antibodies  >4 publications with YWHAG and Cardiovascular Diseases
 Neoplasm Invasiveness Antibodies  >3 publications with YWHAG and Neoplasm Invasiveness
 Lung Neoplasms Antibodies  >3 publications with YWHAG and Lung Neoplasms
 Disease Models, Animal Antibodies  >1 publications with YWHAG and Disease Models, Animal
 Learning Disorders Antibodies  >1 publications with YWHAG and Learning Disorders
 Hypertrophy Antibodies  >1 publications with YWHAG and Hypertrophy

Organs/Tissues associated with anti-YWHAG antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with YWHAG and Brain
 Bone Antibodies  >4 publications with YWHAG and Bone
 Muscle Antibodies  >4 publications with YWHAG and Muscle
 Lung Antibodies  >4 publications with YWHAG and Lung
 Nerve Antibodies  >3 publications with YWHAG and Nerve
 Heart Antibodies  >3 publications with YWHAG and Heart
 Blood Antibodies  >2 publications with YWHAG and Blood
 Bone Marrow Antibodies  >2 publications with YWHAG and Bone Marrow
 Liver Antibodies  >1 publications with YWHAG and Liver
 Kidney Antibodies  >1 publications with YWHAG and Kidney
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