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Ribophorin I (RPN1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2003366
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MBS2003366 is an antibody targeting RPN1. Also known as Polyclonal Antibody to Ribophorin I (RPN1). Reactive in Human, Mouse, Rat, Pig. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ribophorin I (RPN1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Ribophorin I (RPN1)
Product Gene Name
anti-RPN1 antibody
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG S-ASSEAPP LINEDVKRTV DLSSHLAKVT AEVVLAHLGG GSTSRATSFL LALEPELEAR LAHLGVQVKG EDEEENNLEV RETKIKGKSG RFFTVKLPVA LDPGAKISVI VETVYTHVLH PYPTQITQSE KQFVVFEGNH YFYSPYPTKT QTMRVKLAS
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Pig
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against RPN1. It has been selected for its ability to recognize RPN1 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: RPN1 (MBS2003366)
Biotin Conjugated Antibody: RPN1 (MBS2003682)
Matching Pairs
Unconjugated Antibody: RPN1 (MBS2003366)
Immunogen: RPN1 (MBS2009708)
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Molecular Weight
68,569 Da
OMIM
Y00281 mRNA
3D Structure
ModBase 3D Structure for P04843
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant RPN1 (Ala24~Ser179) expressed in E.coli.
Cross Reactivity
Human, Pig
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2068081
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-RPN1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-RPN1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-RPN1 antibody

Western Blot: Sample: Porcine Pancreas lysate; Primary Ab: 2ug/ml Rabbit Anti-Human RPN1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-RPN1 Polyclonal Antibody – Cat# MBS2003366

Western Blot (WB) of anti-RPN1 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-RPN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2003366

Immunohistochemistry (IHC) of anti-RPN1 antibody

DAB staining on IHC-P; Samples: Human Cerebrum Tissue)
Rabbit Anti-RPN1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2003366

Other Notes

Small volumes of anti-RPN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RPN1. It may not necessarily be applicable to this product.

NCBI GI #
4506675
NCBI GeneID
6184
NCBI Accession #
NP_002941.1
UniProt Primary Accession #
P04843
UniProt Secondary Accession #
Q68DT1; B2R5Z0; D3DNB6
UniProt Related Accession #
P04843
GenBank Nucleotide #
NM_002950.3
NCBI Official Full Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
NCBI Official Synonym Full Names
ribophorin I
NCBI Official Symbol
RPN1
NCBI Official Synonym Symbols
OST1; RBPH1
UniProt Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
UniProt Synonym Protein Names
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Ribophorin I; RPN-I; Ribophorin-1
Protein Family
26S proteasome regulatory
UniProt Gene Name
RPN1
UniProt Synonym Gene Names
RPN-I
NCBI Protein Information
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

NCBI Summary for RPN1

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]
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UniProt Comments for RPN1

RPN1: Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Belongs to the OST1 family.

Protein type: EC 2.4.99.18; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; extracellular matrix; integral component of membrane; melanosome; membrane; oligosaccharyltransferase complex; rough endoplasmic reticulum

Molecular Function: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; protein binding; RNA binding

Biological Process: cellular protein modification process; protein N-linked glycosylation via asparagine
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Research Articles on RPN1

1. These results clearly demonstrate that the association of malectin with ribophorin I is required to capture misfolded alpha1-antitrypsin and direct them to the degradation pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPN1 antibody

 Reference Product  PubMed Publications
 RPN2 antibody  >16 publications with RPN1 and RPN2

Pathways associated with anti-RPN1 antibody

 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway antibodies  Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway Diagram
 Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway antibodies  Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway Diagram
 Defective ALG12 Causes ALG12-CDG (CDG-1g) Pathway antibodies  Defective ALG12 Causes ALG12-CDG (CDG-1g) Pathway Diagram
 Defective ALG14 Causes Congenital Myasthenic Syndrome (ALG14-CMS) Pathway antibodies  Defective ALG14 Causes Congenital Myasthenic Syndrome (ALG14-CMS) Pathway Diagram
 Defective ALG2 Causes ALG2-CDG (CDG-1i) Pathway antibodies  Defective ALG2 Causes ALG2-CDG (CDG-1i) Pathway Diagram
 Defective ALG3 Causes ALG3-CDG (CDG-1d) Pathway antibodies  Defective ALG3 Causes ALG3-CDG (CDG-1d) Pathway Diagram
 Defective ALG6 Causes ALG6-CDG (CDG-1c) Pathway antibodies  Defective ALG6 Causes ALG6-CDG (CDG-1c) Pathway Diagram
 Defective ALG8 Causes ALG8-CDG (CDG-1h) Pathway antibodies  Defective ALG8 Causes ALG8-CDG (CDG-1h) Pathway Diagram
 Defective ALG9 Causes ALG9-CDG (CDG-1l) Pathway antibodies  Defective ALG9 Causes ALG9-CDG (CDG-1l) Pathway Diagram

Diseases associated with anti-RPN1 antibody

 Disease Name  Pubmed Publications
 Anemia Antibodies  >3 publications with RPN1 and Anemia
 Necrosis Antibodies  >3 publications with RPN1 and Necrosis
 Lung Diseases Antibodies  >2 publications with RPN1 and Lung Diseases
 Cardiovascular Diseases Antibodies  >2 publications with RPN1 and Cardiovascular Diseases
 Heart Diseases Antibodies  >2 publications with RPN1 and Heart Diseases
 Brain Diseases Antibodies  >1 publications with RPN1 and Brain Diseases
 Heart Failure Antibodies  >1 publications with RPN1 and Heart Failure
 Nerve Degeneration Antibodies  >1 publications with RPN1 and Nerve Degeneration
 Kidney Diseases Antibodies  >1 publications with RPN1 and Kidney Diseases

Organs/Tissues associated with anti-RPN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >9 publications with RPN1 and Bone
 Bone Marrow Antibodies  >9 publications with RPN1 and Bone Marrow
 Blood Antibodies  >6 publications with RPN1 and Blood
 Muscle Antibodies  >4 publications with RPN1 and Muscle
 Heart Antibodies  >4 publications with RPN1 and Heart
 Lung Antibodies  >3 publications with RPN1 and Lung
 Kidney Antibodies  >2 publications with RPN1 and Kidney
 Nerve Antibodies  >2 publications with RPN1 and Nerve
 Brain Antibodies  >2 publications with RPN1 and Brain
 Eye Antibodies  >1 publications with RPN1 and Eye
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