• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ACTA2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200339

Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified.… More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ACTA2, Monoclonal Antibody
Full Product Name
ACTA2 antibody
Product Gene Name
anti-ACTA2 antibody
Product Synonym Names
Actin aortic smooth muscle; Actin; aortic smooth muscle; AAT6; ACTSA; MYMY5
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT132D3
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
42,009 Da
OMIM
X13839 mRNA
3D Structure
ModBase 3D Structure for P62736
Antigen Species
Human
Immunogen
Recombinant human ACTA2 (3-377aa) purified from E. coli
Clone Note
Anti-human ACTA2 mAb, clone AT132D3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACTA2 protein.

Product Categories/Family for anti-ACTA2 antibody

Cytoskeleton; Actin & Myosin Related
Applications Tested/Suitable for anti-ACTA2 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ACTA2 antibody
WB: 1:1000.

Western Blot (WB) of anti-ACTA2 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: SW480 cell lysate Lane 3.: HeLa cell lysate
Mouse Anti-ACTA2 Monoclonal Antibody – Cat# MBS200339

Immunofluorescence (IF) of anti-ACTA2 antibody

ICC/IF analysis of ACTA2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACTA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-ACTA2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200339

Product References and Citations for anti-ACTA2 antibody

Herman IM. et al. (1993) Curr Opin Cell Biol. 5 (1): 48-55.; Guo DC., et al. (2009) Am J Hum Genet. 84 (5): 617-627.

Other Notes

Small volumes of anti-ACTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTA2 purchase

MBS200339 is an antibody targeting ACTA2. Also known as ACTA2 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTA2. It may not necessarily be applicable to this product.

NCBI GI #
4501883
NCBI GeneID
59
NCBI Accession #
NP_001604
UniProt Primary Accession #
P62736
UniProt Secondary Accession #
P03996; P04108; Q6FI19; B2R8A4
UniProt Related Accession #
P62736
GenBank Nucleotide #
NM_001613.3
NCBI Official Full Name
actin, aortic smooth muscle
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2
NCBI Official Synonym Symbols
ACTSA
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Synonym Protein Names
Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Protein Family
Actin
UniProt Gene Name
ACTA2
UniProt Synonym Gene Names
ACTSA; ACTVS
NCBI Protein Information
actin, aortic smooth muscle

NCBI Summary for ACTA2

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]
Show more

UniProt Comments for ACTA2

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Research Articles on ACTA2

1. our results seemed to justify the conclusion that ACTA2 did not play a significant role in the pathogenesis of BAV aortopathy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ACTA2 antibody

 Reference Product  PubMed Publications
 MYH11 antibody  >56 publications with ACTA2 and MYH11
 SRF antibody  >19 publications with ACTA2 and SRF
 TPM3 antibody  >8 publications with ACTA2 and TPM3
 LMOD1 antibody  >2 publications with ACTA2 and LMOD1
 TPM2 antibody  >2 publications with ACTA2 and TPM2
 TPM1 antibody  >2 publications with ACTA2 and TPM1
 MYL9 antibody  >1 publications with ACTA2 and MYL9

Pathways associated with anti-ACTA2 antibody

 Products by Pathway  Pathway Diagram
 Apelin Signaling Pathway antibodies  Apelin Signaling Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Smooth Muscle Contraction Pathway antibodies  Smooth Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram

Diseases associated with anti-ACTA2 antibody

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >497 publications with ACTA2 and Fibrosis
 Inflammation Antibodies  >151 publications with ACTA2 and Inflammation
 Liver Diseases Antibodies  >149 publications with ACTA2 and Liver Diseases
 Pulmonary Fibrosis Antibodies  >111 publications with ACTA2 and Pulmonary Fibrosis
 Liver Cirrhosis Antibodies  >110 publications with ACTA2 and Liver Cirrhosis
 Kidney Diseases Antibodies  >77 publications with ACTA2 and Kidney Diseases
 Heart Diseases Antibodies  >73 publications with ACTA2 and Heart Diseases
 Aortic Aneurysm, Thoracic Antibodies  >59 publications with ACTA2 and Aortic Aneurysm, Thoracic
 Necrosis Antibodies  >57 publications with ACTA2 and Necrosis
 Breast Neoplasms Antibodies  >48 publications with ACTA2 and Breast Neoplasms

Organs/Tissues associated with anti-ACTA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1097 publications with ACTA2 and Muscle
 Vascular Antibodies  >340 publications with ACTA2 and Vascular
 Blood Antibodies  >244 publications with ACTA2 and Blood
 Lung Antibodies  >206 publications with ACTA2 and Lung
 Liver Antibodies  >188 publications with ACTA2 and Liver
 Connective Tissue Antibodies  >178 publications with ACTA2 and Connective Tissue
 Eye Antibodies  >171 publications with ACTA2 and Eye
 Kidney Antibodies  >135 publications with ACTA2 and Kidney
 Skin Antibodies  >123 publications with ACTA2 and Skin
 Heart Antibodies  >101 publications with ACTA2 and Heart
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000