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Peroxiredoxin 5 (PRDX5) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200347

Peroxiredoxin 5, also known as PRDX5, is a member of the peroxiredoxin family of antioxidant enzymes, which reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. This protein may play an… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Peroxiredoxin 5 (PRDX5), Monoclonal Antibody
Full Product Name
Peroxiredoxin 5 antibody
Product Gene Name
Product Synonym Names
Peroxiredoxin 5; ACR1; AOEB116; B116; PLP; PMP20; PRDX5; PRXV; SBBI10; AOEB166; PRDX 5; Thioredoxin reductase.
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT6A10
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
12,897 Da
OMIM
AF231705 mRNA
3D Structure
ModBase 3D Structure for P30044
Antigen Species
Human
Immunogen
Recombinant human Peroxiredoxin 5 (53-214aa) purified from E. coli
Clone Note
Anti-human Peroxiredoxin 5 mAb, clone AT6A10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PRDX5 protein.

Product Categories/Family for anti-PRDX5 antibody

Applications Tested/Suitable for anti-PRDX5 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-PRDX5 antibody

The Recombinant Human PRDX5 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin 5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant PRDX5 protein Lane 2. : MCF-7 cell lysate Lane 3. : A549 cell lysate Lane 4. : HeLa cell lysate Lane 5. : 293T cell lysate Lane 6. : Jurkat cell lysate
Mouse Anti-PRDX5 Monoclonal Antibody – Cat# MBS200347

Immunofluorescence (IF) of anti-PRDX5 antibody

ICC/IF analysis of PRDX5 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-PRDX5 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200347

Product References and Citations for anti-PRDX5 antibody

Yamashita H., et al. (1999) J Biol Chem. 274 (42): 29897-29904. ; Declercg JP., et al. (2001) J Mol Biol. 311 (4): 751-759.

Other Notes

Small volumes of anti-PRDX5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRDX5 purchase

MBS200347 is an antibody targeting PRDX5. Also known as Peroxiredoxin 5 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PRDX5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
peroxiredoxin-5, mitochondrial isoform L
NCBI Official Synonym Full Names
peroxiredoxin 5
NCBI Official Symbol
PRDX5
NCBI Official Synonym Symbols
PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55
UniProt Protein Name
Peroxiredoxin-5, mitochondrial
UniProt Synonym Protein Names
Alu corepressor 1; Antioxidant enzyme B166; AOEB166; Liver tissue 2D-page spot 71B; PLP; Peroxiredoxin V; Prx-V; Peroxisomal antioxidant enzyme; TPx type VI; Thioredoxin peroxidase PMP20
Protein Family
UniProt Gene Name
PRDX5
UniProt Synonym Gene Names
ACR1; AOEB166; Prx-V
NCBI Protein Information
peroxiredoxin-5, mitochondrial

NCBI Summary for PRDX5

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein interacts with peroxisome receptor 1 and plays an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. The use of alternate transcription start sites is thought to result in transcript variants that use different in-frame translational start codons to generate isoforms that are targeted to the mitochondrion (isoform L) or peroxisome/cytoplasm (isoform S). Multiple related pseudogenes have been defined for this gene. [provided by RefSeq, Nov 2017]
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UniProt Comments for PRDX5

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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