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GNMT Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200351

Ras-related protein Glycine N-methyltransferase (GNMT) catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GNMT, Monoclonal Antibody
Full Product Name
GNMT antibody
Product Gene Name
Product Synonym Names
Glycine N-methyltransferase; GNMT; Glycine N-methyltransferase; Glycine N-methyltransferase EC 2.1.1.20; Glycine N methyltransferase.
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT5D1
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
32,742 Da
OMIM
AF101477 mRNA
3D Structure
ModBase 3D Structure for Q14749
Antigen Species
Human
Immunogen
Recombinant human GNMT (1-295aa) purified from E. coli
Clone Note
Anti-human GNMT mAb, clone AT5D1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GNMT protein

Product Categories/Family for anti-GNMT antibody

Applications Tested/Suitable for anti-GNMT antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Western Blot (WB) of anti-GNMT antibody

The Recombinant protein (50ng) and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : GNMT recombinant protein Lane 2. : Liver tissue lysate The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : GNMT Transfected 293T cell lysate
Mouse Anti-GNMT Monoclonal Antibody – Cat# MBS200351

Immunofluorescence (IF) of anti-GNMT antibody

ICC/IF analysis of GNMT in HepG2 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GNMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-GNMT Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200351

Product References and Citations for anti-GNMT antibody

Velichkova., et al. (2006) J Phys Chem B. 109: 8216-8219.; Huang YC., et al. (2008) J Gastroenterol Hepatol. 23: 1384.

Other Notes

Small volumes of anti-GNMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GNMT purchase

MBS200351 is an antibody targeting GNMT. Also known as GNMT antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GNMT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glycine N-methyltransferase isoform 1
NCBI Official Synonym Full Names
glycine N-methyltransferase
NCBI Official Symbol
GNMT
NCBI Official Synonym Symbols
HEL-S-182mP
UniProt Protein Name
Glycine N-methyltransferase
UniProt Gene Name
GNMT
NCBI Protein Information
glycine N-methyltransferase

NCBI Summary for GNMT

The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. This protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). Alternative splicing results in multiple transcript variants. Naturally occurring readthrough transcription occurs between the upstream CNPY3 (canopy FGF signaling regulator 3) gene and this gene and is represented with GeneID:107080644. [provided by RefSeq, Jan 2016]
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UniProt Comments for GNMT

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GNMT antibody

 Reference Product  PubMed Publications
 SHMT1 antibody  >1 publications with GNMT and SHMT1
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