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LDHB Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200353

LDHB, also known as L-lactate dehydrogenase B chain, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can… More details

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Product Overview

Online Datasheet in PDF
Product Name
LDHB, Monoclonal Antibody
Full Product Name
LDHB antibody
Product Gene Name
Product Synonym Names
L-lactate dehydrogenase B chain; LDH-H; TRG-5.; L-lactate dehydrogenase B chain; LDH-B; LDH heart subunit; Renal carcinoma antigen NY-REN-46
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT14D7
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
36,638 Da
3D Structure
ModBase 3D Structure for P07195
Antigen Species
Human
Immunogen
Recombinant human LDHB (1-334aa) purified from E. coli
Clone Note
Anti-human LDHB mAb, clone AT14D7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human LDHB protein.

Product Categories/Family for anti-LDHB antibody

Applications Tested/Suitable for anti-LDHB antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-LDHB antibody

The Recombinant Human LDHB (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LDHB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant LDHB protein Lane 2. : A549 cell lysate Lane 3. : 293T cell lysate Lane 4. : K562 cell lysate Lane 5. : Jurkat cell lysate
Mouse Anti-LDHB Monoclonal Antibody – Cat# MBS200353

Immunofluorescence (IF) of anti-LDHB antibody

ICC/IF analysis of LDHB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LDHB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-LDHB Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200353

Product References and Citations for anti-LDHB antibody

Read JA., et al. (2001) Proteins. 43 (2): 175-185. ; Butt AA., et al. (2002) AIDS. 12 (7): 317-321.

Other Notes

Small volumes of anti-LDHB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LDHB purchase

MBS200353 is an antibody targeting LDHB. Also known as LDHB antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LDHB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
L-lactate dehydrogenase B chain isoform LDHB
NCBI Official Synonym Full Names
lactate dehydrogenase B
NCBI Official Symbol
LDHB
NCBI Official Synonym Symbols
LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
UniProt Protein Name
L-lactate dehydrogenase B chain
UniProt Synonym Protein Names
LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
UniProt Gene Name
LDHB
UniProt Synonym Gene Names
LDH-B; LDH-H
NCBI Protein Information
L-lactate dehydrogenase B chain

NCBI Summary for LDHB

This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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