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Tubulin Beta 3 (TUBB3) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200355

Tubulin Beta 3 is 450 amino acid protein. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Tubulin Beta 3 (TUBB3), Monoclonal Antibody
Full Product Name
Tubulin Beta 3 antibody
Product Gene Name
anti-TUBB3 antibody
Product Synonym Names
Tubulin beta 3 class III; Tubulin; beta 3 class III; beta-4; CDCBM; CFEOM3A; TuBB4
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT2F5
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
42,433 Da
OMIM
U47634 mRNA
3D Structure
ModBase 3D Structure for Q13509
Antigen Species
Human
Immunogen
Recombinant human TUBB3 (1-450aa) purified from E. coli
Clone Note
Anti-human Tubulin Beta 3 mAb, clone AT2F5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TUBB3 protein.

Product Categories/Family for anti-TUBB3 antibody

Cytoskeleton; Neuroscience
Applications Tested/Suitable for anti-TUBB3 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-TUBB3 antibody
WB: 1:1000.
Recommended dilution range for ICC/IF and Flow Cytometry is 1:200.

Western Blot (WB) of anti-TUBB3 antibody

The Cell lysates (40ug) and Mouse tissue extract (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tubulin Beta 3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: 293T cell lysate Lane 3.: Mouse Brain tissue extract
Mouse Anti-TUBB3 Monoclonal Antibody – Cat# MBS200355

Immunofluorescence (IF) of anti-TUBB3 antibody

ICC/IF analysis of Tubulin Beta 3 in U-87MG cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Tubulin Beta 3 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-TUBB3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200355

Product References and Citations for anti-TUBB3 antibody

Qu C., et al. (2013) J Cell Sci. 126 (14): 3070-3081; Marczak A., et al. (2015) Postepy Hig Med Dosw. 69: 158-164; McCarroll J.A., et al. (2015) Cancer Res. 75 (2): 415-425

Other Notes

Small volumes of anti-TUBB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TUBB3 purchase

MBS200355 is an antibody targeting TUBB3. Also known as Tubulin Beta 3 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TUBB3. It may not necessarily be applicable to this product.

NCBI GI #
50592996
NCBI GeneID
10381
NCBI Accession #
NP_006077
UniProt Primary Accession #
Q13509
UniProt Secondary Accession #
Q9BTZ0; Q9BW10; A8K854
UniProt Related Accession #
Q13509
GenBank Nucleotide #
NM_006086.3
NCBI Official Full Name
tubulin beta-3 chain isoform 1
NCBI Official Synonym Full Names
tubulin beta 3 class III
NCBI Official Symbol
TUBB3
NCBI Official Synonym Symbols
CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
UniProt Protein Name
Tubulin beta-3 chain
UniProt Synonym Protein Names
Tubulin beta-4 chain; Tubulin beta-III
Protein Family
Tubulin
UniProt Gene Name
TUBB3
UniProt Synonym Gene Names
TUBB4
NCBI Protein Information
tubulin beta-3 chain

NCBI Summary for TUBB3

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
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UniProt Comments for TUBB3

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Research Articles on TUBB3

1. The authors found that transient TUBB3 activation, through ABCB1, in response to the stimulation of FOXO3a expression, significantly contributes to the cross-resistance of the paclitaxel-resistant cell population and consequently limits the efficacy of both agents where cancer cells have developed multiple resistance.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TUBB3 antibody

 Reference Product  PubMed Publications
 TUBA1A antibody  >19 publications with TUBB3 and TUBA1A
 KIF21A antibody  >15 publications with TUBB3 and KIF21A
 TUBA8 antibody  >8 publications with TUBB3 and TUBA8

Pathways associated with anti-TUBB3 antibody

 Products by Pathway  Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway antibodies  Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway Diagram
 Diurnally Regulated Genes With Circadian Orthologs Pathway antibodies  Diurnally Regulated Genes With Circadian Orthologs Pathway Diagram
 Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway antibodies  Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Parkin-Ubiquitin Proteasomal System Pathway antibodies  Parkin-Ubiquitin Proteasomal System Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with anti-TUBB3 antibody

 Disease Name  Pubmed Publications
 Fibrosis Of Extraocular Muscles, Congenital, 3A, with or without Extraocular Involvement Antibodies  >644 publications with TUBB3 and Fibrosis Of Extraocular Muscles, Congenital, 3A, with or without Extraocular Involvement
 Nervous System Diseases Antibodies  >113 publications with TUBB3 and Nervous System Diseases
 Brain Diseases Antibodies  >63 publications with TUBB3 and Brain Diseases
 Breast Neoplasms Antibodies  >43 publications with TUBB3 and Breast Neoplasms
 Ovarian Neoplasms Antibodies  >32 publications with TUBB3 and Ovarian Neoplasms
 Fibrosis Antibodies  >28 publications with TUBB3 and Fibrosis
 Disease Models, Animal Antibodies  >27 publications with TUBB3 and Disease Models, Animal
 Nerve Degeneration Antibodies  >14 publications with TUBB3 and Nerve Degeneration
 Cardiovascular Diseases Antibodies  >13 publications with TUBB3 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >11 publications with TUBB3 and Neoplasms, Experimental

Organs/Tissues associated with anti-TUBB3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >185 publications with TUBB3 and Brain
 Nerve Antibodies  >131 publications with TUBB3 and Nerve
 Lung Antibodies  >84 publications with TUBB3 and Lung
 Eye Antibodies  >55 publications with TUBB3 and Eye
 Muscle Antibodies  >40 publications with TUBB3 and Muscle
 Embryonic Tissue Antibodies  >38 publications with TUBB3 and Embryonic Tissue
 Blood Antibodies  >38 publications with TUBB3 and Blood
 Bone Antibodies  >31 publications with TUBB3 and Bone
 Ganglia Antibodies  >27 publications with TUBB3 and Ganglia
 Bone Marrow Antibodies  >20 publications with TUBB3 and Bone Marrow
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