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S100A4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200356

S100 calcium-binding protein A4 (S100A4) is member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of… More details

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Product Overview

Online Datasheet in PDF
Product Name
S100A4, Monoclonal Antibody
Full Product Name
S100A4 antibody
Product Gene Name
Product Synonym Names
S100 calcium binding protein A4; 18A2; 42a; Capl; FSp1; metastasin; Mts1; PeL98; pk9a
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1C3
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
11,729 Da
OMIM
M80563 mRNA
3D Structure
ModBase 3D Structure for P26447
Antigen Species
Human
Immunogen
Recombinant human S100A4 (1-101aa) purified from E. coli
Clone Note
Anti-human S100A4 mAb, clone AT1C3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human S100A4 protein.

Product Categories/Family for anti-S100A4 antibody

Applications Tested/Suitable for anti-S100A4 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-S100A4 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : A549 cell lysate Lane 3. : NIH/3T3 cell lysate Lane 4. : 3T3-L1 cell lysate
Mouse Anti-S100A4 Monoclonal Antibody – Cat# MBS200356

Immunofluorescence (IF) of anti-S100A4 antibody

ICC/IF analysis of S100A4 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human S100A4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of S100A4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining, Wheat germ agglutinin (WGA) with fluorescein conjugate (Red) for plasma membrane staining and monoclonal anti-human S100A4 antibody (1:500) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-S100A4 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200356

Product References and Citations for anti-S100A4 antibody

Stoler A., et al. (1985) Proc Natl Acad Sci USA. 82 (2): 570-574.; Choudhary C., et al. (2009) Science. 325: 834-840.; Bian Y., et al. (2014) J Proteomics. 96: 253-262.;

Other Notes

Small volumes of anti-S100A4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for S100A4 purchase

MBS200356 is an antibody targeting S100A4. Also known as S100A4 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for S100A4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein S100-A4
NCBI Official Synonym Full Names
S100 calcium binding protein A4
NCBI Official Symbol
S100A4
NCBI Official Synonym Symbols
42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
UniProt Protein Name
Protein S100-A4
UniProt Synonym Protein Names
Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; S100 calcium-binding protein A4
Protein Family
UniProt Gene Name
S100A4
UniProt Synonym Gene Names
CAPL; MTS1
NCBI Protein Information
protein S100-A4

NCBI Summary for S100A4

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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