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RAS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200363

All RAS protein family members belong to a class of protein called small GTPase, and are involved cellular signal transduction. RAS is 'switched on' by incoming signals, it subsequently switches on other proteins, which ultimately turn on genes involved in… More details

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Product Overview

Online Datasheet in PDF
Product Name
RAS, Monoclonal Antibody
Full Product Name
RAS antibody
Product Gene Name
anti-RAS antibody
Product Synonym Names
GTPase Kras; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; NS3; RASK2; GTPase Kras,K-RAS2A; K RAS2B; K RAS4A; K RAS4B; KI RAS; KRAS 1; NS 3
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT2F8
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (determined by BCA assay) (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week.
For long term storage, aliquot and store at -20 degree C to -80 degree C.
Avoid repeated freezing and thawing cycles.
3D Structure
ModBase 3D Structure for P01116
Immunogen
Recombinant human KRAS (1-186aa) purified from E. coli

Product Categories/Family for anti-RAS antibody

Signal Transduction
Applications Tested/Suitable for anti-RAS antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-RAS antibody
This antibody has been tested by ELISA, Western Blot, and ICC/IF analysis to assure specificity and reactivity. Since applications vary, however, each application should be titrated by the reagent to obtain optimal results.

Western Blot (WB) of anti-RAS antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : HepG2 cell lysate
Lane 3. : Ramos cell lysate
Lane 4. : A549 cell lysate
Lane 5. : Balb/3T3 cell lysate
Mouse Anti-RAS Monoclonal Antibody – Cat# MBS200363

Western Blot (WB) of anti-RAS antibody

The Recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : KRAS recombinant protein (MBS205184)
Lane 2. : HRAS recombinant protein (MBS203609)
Lane 3. : NRAS recombinant protein (203546)
Mouse Anti-RAS Monoclonal Antibody, Western Blot (WB) – Cat# MBS200363

Immunofluorescence (IF) of anti-RAS antibody

ICC/IF analysis of RAS in HeLa cells. The cell was stained with MBS200363 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-RAS Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200363

Product References and Citations for anti-RAS antibody

McGrath JP., et al. (1983) Nature. 304 (5926): 501-506.; Schubbert S., et al. (2006) Nat Genet. 38 (3): 331-336.

Other Notes

Small volumes of anti-RAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAS purchase

MBS200363 is an antibody targeting RAS. Also known as RAS antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RAS. It may not necessarily be applicable to this product.

NCBI GI #
15718763
NCBI GeneID
3845
NCBI Accession #
NP_203524
UniProt Primary Accession #
P01116
UniProt Secondary Accession #
P01118; Q96D10; A8K8Z5; B0LPF9
UniProt Related Accession #
P01116
GenBank Nucleotide #
NM_033360.3
NCBI Official Full Name
GTPase KRas isoform a
NCBI Official Synonym Full Names
KRAS proto-oncogene, GTPase
NCBI Official Symbol
KRAS
NCBI Official Synonym Symbols
NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
UniProt Protein Name
GTPase KRas
UniProt Synonym Protein Names
K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras
Protein Family
Rosmarinate synthase
UniProt Gene Name
KRAS
UniProt Synonym Gene Names
KRAS2; RASK2
NCBI Protein Information
GTPase KRas

NCBI Summary for RAS

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
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UniProt Comments for RAS

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306).
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Research Articles on RAS

1. Reduced insulin receptor substrate-1 (IRS-1) staining in lung adenocarcinoma tissue microarray displayed a significant survival disadvantage, especially within the Kirsten rat sarcoma viral oncogene homolog (KRAS) mutant subgroup.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RAS antibody

 Reference Product  PubMed Publications
 EGFR antibody  >3075 publications with RAS and EGFR
 BRAF antibody  >2824 publications with RAS and BRAF
 PIK3CA antibody  >1203 publications with RAS and PIK3CA
 ERBB2 antibody  >403 publications with RAS and ERBB2
 MAP2K1 antibody  >115 publications with RAS and MAP2K1
 RAF1 antibody  >88 publications with RAS and RAF1
 SOS1 antibody  >82 publications with RAS and SOS1
 MAPK1 antibody  >39 publications with RAS and MAPK1
 ARAF antibody  >14 publications with RAS and ARAF
 MAPK3 antibody  >8 publications with RAS and MAPK3

Pathways associated with anti-RAS antibody

 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of RAS In B Cells Pathway antibodies  Activation Of RAS In B Cells Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram

Diseases associated with anti-RAS antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >4027 publications with RAS and Carcinoma
 Colorectal Neoplasms Antibodies  >3172 publications with RAS and Colorectal Neoplasms
 Adenocarcinoma Antibodies  >2930 publications with RAS and Adenocarcinoma
 Lung Neoplasms Antibodies  >2157 publications with RAS and Lung Neoplasms
 Carcinogenesis Antibodies  >1196 publications with RAS and Carcinogenesis
 Carcinoma, Non-Small-Cell Lung Antibodies  >1110 publications with RAS and Carcinoma, Non-Small-Cell Lung
 Pancreatic Neoplasms Antibodies  >1108 publications with RAS and Pancreatic Neoplasms
 Neoplasm Metastasis Antibodies  >1003 publications with RAS and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >590 publications with RAS and Neoplasms, Experimental
 Colonic Neoplasms Antibodies  >533 publications with RAS and Colonic Neoplasms

Organs/Tissues associated with anti-RAS antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >2747 publications with RAS and Lung
 Blood Antibodies  >833 publications with RAS and Blood
 Liver Antibodies  >659 publications with RAS and Liver
 Pancreas Antibodies  >528 publications with RAS and Pancreas
 Vascular Antibodies  >346 publications with RAS and Vascular
 Skin Antibodies  >317 publications with RAS and Skin
 Lymph Antibodies  >317 publications with RAS and Lymph
 Lymph Node Antibodies  >311 publications with RAS and Lymph Node
 Bone Antibodies  >229 publications with RAS and Bone
 Intestine Antibodies  >221 publications with RAS and Intestine
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