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PGK1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200372

Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a… More details

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Product Overview

Online Datasheet in PDF
Product Name
PGK1, Monoclonal Antibody
Full Product Name
PGK1 antibody
Product Gene Name
anti-PGK1 antibody
Product Synonym Names
Phosphoglycerate kinase 1; Pgk-1
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
AT2F4
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (determined by BCA assay) (lot specific)
Preparation and Storage
Can be stored at 2°C to 8°C for one week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
OMIM
V00572 mRNA
3D Structure
ModBase 3D Structure for P00558
Immunogen
Recombinant human PGK1 (1-417aa) purified from E. coli

Product Categories/Family for anti-PGK1 antibody

Signal Transduction; Metabolism
Applications Tested/Suitable for anti-PGK1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC)
Application Notes for anti-PGK1 antibody
The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Western Blot (WB) of anti-PGK1 antibody

Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : LNCaP cell lysate
Lane 3. : A549 cell lysate
Lane 4. : HepG2 cell lysate
Lane 5. : 293T cell lysate
Lane 6. : K562 cell lysate
Lane 7. : Jurkat cell lysate
Mouse Anti-PGK1 Monoclonal Antibody – Cat# MBS200372

Immunofluorescence (IF) of anti-PGK1 antibody

ICC/IF analysis of PGK1 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-PGK1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200372

Product References and Citations for anti-PGK1 antibody

Svaasand EK., et al. (2007) Muscle Nerve. 36 (5): 679-684. ; Valentin C., et al. (1998) Hum Mutat. 12 (4): 280-287.

Other Notes

Small volumes of anti-PGK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGK1 purchase

MBS200372 is an antibody targeting PGK1. Also known as PGK1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PGK1. It may not necessarily be applicable to this product.

NCBI GI #
4505763
NCBI GeneID
5230
NCBI Accession #
NP_000282
UniProt Primary Accession #
P00558
UniProt Secondary Accession #
Q5J7W1; Q6IBT6; Q8NI87; A8K4W6; B7Z7A9
UniProt Related Accession #
P00558
GenBank Nucleotide #
NM_000291.3
NCBI Official Full Name
phosphoglycerate kinase 1
NCBI Official Synonym Full Names
phosphoglycerate kinase 1
NCBI Official Symbol
PGK1
NCBI Official Synonym Symbols
PGKA; MIG10; HEL-S-68p
UniProt Protein Name
Phosphoglycerate kinase 1
UniProt Synonym Protein Names
Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2
Protein Family
Phosphoglycerate kinase
UniProt Gene Name
PGK1
UniProt Synonym Gene Names
PGKA; PRP 2
NCBI Protein Information
phosphoglycerate kinase 1

NCBI Summary for PGK1

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]
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UniProt Comments for PGK1

In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).

Research Articles on PGK1

1. Data show that LINC00963 (MetaLnc9) interacted with the glycolytic kinase PGK1 and prevented its ubiquitination in non-small cell lung cancer (NSCLC) cells, leading to activation of the oncogenic AKT/mTOR signaling pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PGK1 antibody

 Reference Product  PubMed Publications
 GAPDH antibody  >69 publications with PGK1 and GAPDH
 ENO1 antibody  >20 publications with PGK1 and ENO1
 TPI1 antibody  >10 publications with PGK1 and TPI1
 ENO2 antibody  >7 publications with PGK1 and ENO2
 PGAM1 antibody  >4 publications with PGK1 and PGAM1
 PKM antibody  >1 publications with PGK1 and PKM
 PGAM2 antibody  >1 publications with PGK1 and PGAM2
 ENO3 antibody  >1 publications with PGK1 and ENO3
 GAPDHS antibody  >1 publications with PGK1 and GAPDHS

Pathways associated with anti-PGK1 antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Cori Cycle Pathway antibodies  Cori Cycle Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram

Diseases associated with anti-PGK1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >42 publications with PGK1 and Nervous System Diseases
 Anemia Antibodies  >20 publications with PGK1 and Anemia
 Inflammation Antibodies  >17 publications with PGK1 and Inflammation
 Neoplasms, Experimental Antibodies  >16 publications with PGK1 and Neoplasms, Experimental
 Liver Diseases Antibodies  >15 publications with PGK1 and Liver Diseases
 Breast Neoplasms Antibodies  >13 publications with PGK1 and Breast Neoplasms
 Liver Neoplasms Antibodies  >11 publications with PGK1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >11 publications with PGK1 and Carcinoma, Hepatocellular
 Lung Neoplasms Antibodies  >10 publications with PGK1 and Lung Neoplasms
 Cardiovascular Diseases Antibodies  >10 publications with PGK1 and Cardiovascular Diseases

Organs/Tissues associated with anti-PGK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >55 publications with PGK1 and Blood
 Brain Antibodies  >42 publications with PGK1 and Brain
 Liver Antibodies  >34 publications with PGK1 and Liver
 Muscle Antibodies  >26 publications with PGK1 and Muscle
 Bone Antibodies  >23 publications with PGK1 and Bone
 Lung Antibodies  >20 publications with PGK1 and Lung
 Vascular Antibodies  >17 publications with PGK1 and Vascular
 Skin Antibodies  >14 publications with PGK1 and Skin
 Prostate Antibodies  >13 publications with PGK1 and Prostate
 Bone Marrow Antibodies  >12 publications with PGK1 and Bone Marrow
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