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GSTT1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200376

Glutathione S-transferase theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes (alpha, mu, pi,… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GSTT1, Monoclonal Antibody
Full Product Name
GSTT1 antibody
Product Gene Name
Product Synonym Names
Glutathione S-transferase theta 1; Glutathione S-transferase theta 1; GSTT1; GST class-theta-1; Glutathione transferase T1-1; Glutathione S-transferase theta 1 EC 2.5.1.18; Glutathione S transferase 5; Glutathione S transferase theta 1; GST class theta 1; Glutathione transferase T1 1; GST 5 5; GST CL1
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT38D11
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
OMIM
X79389 mRNA
Antigen Species
Human
Immunogen
Recombinant human GSTT1 (1-240aa) purified from E. coli
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Product Categories/Family for anti-GSTT1 antibody

Applications Tested/Suitable for anti-GSTT1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Western Blot (WB) of anti-GSTT1 antibody

The recombinant human GSTT1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSTT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: GSTT1 Recombinant protein Lane 2.: HeLa cell lysate Lane 3.: 293T cell lysate Lane 4.: Jurkat cell lysate Lane 5.: HL-60 cell lysate
Mouse Anti-GSTT1 Monoclonal Antibody – Cat# MBS200376

Immunofluorescence (IF) of anti-GSTT1 antibody

ICC/IF analysis of GSTT1 in HepG2 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSTT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-GSTT1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200376

Flow Cytommetry (FC) of anti-GSTT1 antibody

Flow cytometry analysis of GSTT1 in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
Mouse Anti-GSTT1 Monoclonal Antibody, Flow Cytommetry (FC) – Cat# MBS200376

Product References and Citations for anti-GSTT1 antibody

Webb G., et al. (1996) Genomics. 33 (1): 121-123.; Mainwaring GW., et al. (1996) Biochem J. 318: 297-303.

Other Notes

Small volumes of anti-GSTT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GSTT1 purchase

MBS200376 is an antibody targeting GSTT1. Also known as GSTT1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GSTT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Glutathione S-transferase theta 1
NCBI Official Synonym Full Names
glutathione S-transferase theta 1
NCBI Official Symbol
GSTT1
UniProt Protein Name
Glutathione S-transferase theta-1
UniProt Synonym Protein Names
GST class-theta-1; Glutathione transferase T1-1
UniProt Gene Name
GSTT1
NCBI Protein Information
glutathione S-transferase theta-1

NCBI Summary for GSTT1

The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2015]
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UniProt Comments for GSTT1

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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