• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LSM5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200378

LSM5, also known as YER146W, is a 91 amino acid protein belonging to the snRNP Sm family. Localized to the nucleus, LSm5 has been found to play a role in the formation and function of the U6 snRNP. Specifically, LSm5 binds to the 3' end of U6 snRNA and… More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LSM5, Monoclonal Antibody
Full Product Name
LSM5 antibody
Product Gene Name
Product Synonym Names
u6 snRNA-associated Sm-like protein LSm5 isoform a; LSM5 homolog; u6 small nuclear RNA associated (S.cerevisiae); YER146W
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT25F8
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
6,850 Da
OMIM
AJ238097 mRNA
3D Structure
ModBase 3D Structure for Q9Y4Y9
Antigen Species
Human
Immunogen
Recombinant human LSM5 (1-91aa) purified from E. coli
Clone Note
Anti-human LSM5 mAb, clone AT25F8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human LSM5 protein.
Applications Tested/Suitable for anti-LSM5 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-LSM5 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LSM5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : K562 cell lysate Lane 3. : Jurkat cell lysate
Mouse Anti-LSM5 Monoclonal Antibody – Cat# MBS200378

Immunofluorescence (IF) of anti-LSM5 antibody

ICC/IF analysis of LSM5 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LSM5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-LSM5 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200378

Product References and Citations for anti-LSM5 antibody

Salgado-Garrido J., et al. (1999) EMBO J. 18: 3451-3462.; Achsel T., et al. (1999) EMBO J. 18: 5789-5802.; Friesen WJ., et al. (2000) J Biol Chem. 275: 26370-26375.; Eystathioy T., et al. (2002) Arthritis Rheum. 46: 726-734.

Other Notes

Small volumes of anti-LSM5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LSM5 purchase

MBS200378 is an antibody targeting LSM5. Also known as LSM5 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LSM5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
U6 snRNA-associated Sm-like protein LSm5 isoform a
NCBI Official Synonym Full Names
LSM5 homolog, U6 small nuclear RNA and mRNA degradation associated
NCBI Official Symbol
LSM5
NCBI Official Synonym Symbols
YER146W
UniProt Protein Name
U6 snRNA-associated Sm-like protein LSm5
UniProt Gene Name
LSM5
NCBI Protein Information
U6 snRNA-associated Sm-like protein LSm5

NCBI Summary for LSM5

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Apr 2004]
Show more

UniProt Comments for LSM5

Plays a role in U6 snRNP assembly and function. Binds to the 3' end of U6 snRNA, thereby facilitating formation of the spliceosomal U4/U6 duplex formation in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000