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NNMT Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200391

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NNMT, Monoclonal Antibody
Full Product Name
NNMT antibody
Product Gene Name
Product Synonym Names
Nicotinamide N-methyltransferase; NNMT; Nicotinamide N-methyltransferase EC 2.1.1.1 Nicotinamide N methyltransferase.
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT11G11
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
29,574 Da
OMIM
U08021 mRNA
3D Structure
ModBase 3D Structure for P40261
Antigen Species
Human
Immunogen
Recombinant human NNMT (1-264aa) purified from E. coli
Clone Note
Anti-human NNMT mAb, clone AT11G11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NNMT protein.

Product Categories/Family for anti-NNMT antibody

Applications Tested/Suitable for anti-NNMT antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-NNMT antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A549 cell lysate
Mouse Anti-NNMT Monoclonal Antibody – Cat# MBS200391

Immunofluorescence (IF) of anti-NNMT antibody

ICC/IF analysis of NNMT in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NNMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-NNMT Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200391

Product References and Citations for anti-NNMT antibody

Aksoy S., et al. (1995) Genomics. 29 (3): 555-61.; Gerhard DS., et al. (2004) Genome Res. 14 (10B): 2121-7.; Riederer M., et al. (2009) Atherosclerosis. 204 (2): 412-7.

Other Notes

Small volumes of anti-NNMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NNMT purchase

MBS200391 is an antibody targeting NNMT. Also known as NNMT antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NNMT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nicotinamide N-methyltransferase
NCBI Official Synonym Full Names
nicotinamide N-methyltransferase
NCBI Official Symbol
NNMT
UniProt Protein Name
Nicotinamide N-methyltransferase
UniProt Gene Name
NNMT
NCBI Protein Information
nicotinamide N-methyltransferase

UniProt Comments for NNMT

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NNMT antibody

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