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VTA1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200402

The VTA1 protein is involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
VTA1, Monoclonal Antibody
Full Product Name
VTA1 antibody
Product Gene Name
Product Synonym Names
vacuolar protein sorting-associated protein VTA1 homolog; DRG-1; DRG1; LIP5; SBP1
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT14G10
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
24,553 Da
OMIM
AF271994 mRNA
3D Structure
ModBase 3D Structure for Q9NP79
Antigen Species
Human
Immunogen
Recombinant human VTA1 (1-307aa) purified from E. coli
Clone Note
Anti-human VTA1 mAb, clone AT14G10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human VTA1 protein.

Product Categories/Family for anti-VTA1 antibody

Applications Tested/Suitable for anti-VTA1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-VTA1 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VTA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: 293T cell lysate Lane 3.: HeLa cell lysate Lane 4.: MCF7 cell lysate Lane 5.: A549 cell lysate Lane 6.: Jurkat cell lysate Lane 7.: K562 cell lysate Lane 8.: LnCaP cell lysate Lane 9.: U937 cell lysate Lane 10.: A431 cell lysate
Mouse Anti-VTA1 Monoclonal Antibody – Cat# MBS200402

Immunofluorescence (IF) of anti-VTA1 antibody

ICC/IF analysis of VTA1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VTA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-VTA1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200402

Product References and Citations for anti-VTA1 antibody

Jun Shi., et al. (2001) Brain Res. 910 (1-2): 29-37. ; Fujita H., et al. (2004) J Cell Sci. 117 (Pt 14): 2997-3009.; Ward DM., et al. (2005) J Biol Chem. 280 (11): 10548-55.;

Other Notes

Small volumes of anti-VTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VTA1 purchase

MBS200402 is an antibody targeting VTA1. Also known as VTA1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for VTA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
vacuolar protein sorting-associated protein VTA1 homolog isoform a
NCBI Official Synonym Full Names
vesicle trafficking 1
NCBI Official Symbol
VTA1
NCBI Official Synonym Symbols
DRG1; LIP5; SBP1; DRG-1; My012; C6orf55; HSPC228
UniProt Protein Name
Vacuolar protein sorting-associated protein VTA1 homolog
UniProt Synonym Protein Names
Dopamine-responsive gene 1 protein; DRG-1; LYST-interacting protein 5; LIP5; SKD1-binding protein 1; SBP1
UniProt Gene Name
VTA1
UniProt Synonym Gene Names
C6orf55; DRG-1; LIP5; SBP1
NCBI Protein Information
vacuolar protein sorting-associated protein VTA1 homolog

NCBI Summary for VTA1

C6ORF55 encodes a protein involved in trafficking of the multivesicular body, an endosomal compartment involved in sorting membrane proteins for degradation in lysosomes (Ward et al., 2005 [PubMed 15644320]).[supplied by OMIM, Mar 2008]

UniProt Comments for VTA1

Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (). Involved in HIV-1 budding.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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