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SOD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200404

Cu/Zn superoxide dismutase (SOD1), is a major antioxidant enzyme that catalyzes the conversion of superoxide anion to hydrogen peroxide and molecular oxygen. SOD1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes… More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SOD1, Monoclonal Antibody
Full Product Name
SOD1 antibody
Product Gene Name
Product Synonym Names
Superoxide dismutase 1 soluble; ALS; SOD; ALS1; IPOA; Cu-Zn superoxide dismutase; Superoxide dismutase 1 soluble; SOD1; Superoxide dismutase 1; soluble ALS 1; Amyotrophic lateral sclerosis 1 Amyotrophic lateral sclerosis 1 adult; Cu/Zn SOD; Cu/Zn superoxide dismutase; Homodimer; Indophenoloxidase A; Mn superoxide dismutase; SOD 1; SOD soluble; SOD2; SODC; Superoxide dismutase 1 soluble; Superoxide dismutase Cu Zn; Superoxide dismutase cystolic
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT6F1
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
15,936 Da
3D Structure
ModBase 3D Structure for P00441
Antigen Species
Human
Immunogen
Recombinant human SOD1 (1-154aa) purified from E. coli
Clone Note
Anti-human SOD1 mAb, clone AT6F1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SOD1 protein.

Product Categories/Family for anti-SOD1 antibody

Applications Tested/Suitable for anti-SOD1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-SOD1 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SOD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : A431 cell lysate Lane 3. : 293T cell lysate Lane 4. : PC3 cell lysate Lane 5. : U87MG cell lysate The recombinant protein (20ng) of SOD two isoform were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SOD1 (1:1000). Lane 1 : Recombinant protein SOD1 Lane 2 : Recombinant protein SOD2
Mouse Anti-SOD1 Monoclonal Antibody – Cat# MBS200404

Immunofluorescence (IF) of anti-SOD1 antibody

ICC/IF analysis of SOD1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SOD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-SOD1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200404

Product References and Citations for anti-SOD1 antibody

Deng HX., et al. (1993) Science. 261 (5124): 1047-51.; Valentine JS., et al. (2003) Proc Natl Acad Sci USA. 100 (7): 3617-22.; Rosen DR., et al. (1993) Nature. 362 (6415): 59-62.;

Other Notes

Small volumes of anti-SOD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SOD1 purchase

MBS200404 is an antibody targeting SOD1. Also known as SOD1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SOD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 1
NCBI Official Symbol
SOD1
NCBI Official Synonym Symbols
ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
UniProt Protein Name
Superoxide dismutase [Cu-Zn]
UniProt Synonym Protein Names
Superoxide dismutase 1; hSod1
Protein Family
UniProt Gene Name
SOD1
UniProt Synonym Gene Names
hSod1
NCBI Protein Information
superoxide dismutase [Cu-Zn]

NCBI Summary for SOD1

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for SOD1

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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