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CAPG Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200419

Macrophage-capping protein, also known as CAPG, belongs to the gelsolin/villin family of actin-regulatory proteins. CAPG is a calcium-sensitive DNA-binding protein that plays a role in macrophage function. It is expressed in macrophages and macrophage-like… More details

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Product Overview

Online Datasheet in PDF
Product Name
CAPG, Monoclonal Antibody
Full Product Name
CAPG antibody
Product Gene Name
Product Synonym Names
Macrophage-capping protein; MCP; AFCP; Macrophage-capping protein Actin capping protein GCAP39; Actin regulatory protein CAP G; CAPG; Capping protein (actin filament) gelsolin like; Capping protein gelsolin like; gCap39; Gelsolin like capping protein; Macrophage capping protein; mbh1; Myc basic motif homolog 1.
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1D10
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
36,857 Da
OMIM
M94345 mRNA
Antigen Species
Human
Immunogen
Recombinant human CAPG (1-348aa) purified from E. coli
Clone Note
Anti-human CAPG mAb, clone AT1D10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CAPG protein.

Product Categories/Family for anti-CAPG antibody

Applications Tested/Suitable for anti-CAPG antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-CAPG antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate
Mouse Anti-CAPG Monoclonal Antibody – Cat# MBS200419

Immunofluorescence (IF) of anti-CAPG antibody

ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-CAPG Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200419

Product References and Citations for anti-CAPG antibody

Kumar A., et al. (2015) Mol Cell Endocrinol. 404: 91-101.; Dabiri G A., et al. (1992) J Biol Chem. 267: 16545-16552.;

Other Notes

Small volumes of anti-CAPG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CAPG purchase

MBS200419 is an antibody targeting CAPG. Also known as CAPG antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CAPG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Capping protein (actin filament), gelsolin-like
NCBI Official Synonym Full Names
capping actin protein, gelsolin like
NCBI Official Symbol
CAPG
NCBI Official Synonym Symbols
MCP; AFCP; HEL-S-66
UniProt Protein Name
Macrophage-capping protein
UniProt Synonym Protein Names
Actin regulatory protein CAP-G
UniProt Gene Name
CAPG
UniProt Synonym Gene Names
AFCP; MCP
NCBI Protein Information
macrophage-capping protein

NCBI Summary for CAPG

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2012]
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UniProt Comments for CAPG

Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CAPG antibody

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