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ADRM1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200431

ADRM1, also known as proteasomal ubiquitin receptor ADRM1, functions as a proteasomal ubiquitin receptor. This protein is an integral plasma membrane protein which promotes cell adhesion. ADRM1 is thought to undergo O-linked glycosylation. Expression of this… More details

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Product Overview

Online Datasheet in PDF
Product Name
ADRM1, Monoclonal Antibody
Full Product Name
ADRM1 antibody
Product Gene Name
Product Synonym Names
Proteasomal ubiquitin receptor ADRM1; ARM1; GP110; Rpn13
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT34C2
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
42,153 Da
OMIM
D64154 mRNA
3D Structure
ModBase 3D Structure for Q16186
Antigen Species
Human
Immunogen
Recombinant human ADRM1 (1-407aa) purified from E. coli
Clone Note
Anti-human ADRM1 mAb, clone AT34C2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ADRM1 protein.
Applications Tested/Suitable for anti-ADRM1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-ADRM1 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADRM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : MCF7 cell lysate Lane 2 : HeLa cell lysate Lane 3 : K562 cell lysate Lane 4 : PC3 cell lysate Lane 5 : 293T cell lysate Lane 6 : HepG2 cell lysate Lane 7 : Jurkat cell lysate Lane 8 : U87MG cell lysate
Mouse Anti-ADRM1 Monoclonal Antibody – Cat# MBS200431

Immunofluorescence (IF) of anti-ADRM1 antibody

ICC/IF analysis of ADRM1 in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ADRM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-ADRM1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200431

Product References and Citations for anti-ADRM1 antibody

Hamazaki J., et al. (2006) EMBO J. 25: 4524-4536. ; Qiu X.-B., et al. (2006) EMBO J. 25: 5742-5753.

Other Notes

Small volumes of anti-ADRM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADRM1 purchase

MBS200431 is an antibody targeting ADRM1. Also known as ADRM1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ADRM1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
proteasomal ubiquitin receptor ADRM1 isoform 1
NCBI Official Synonym Full Names
adhesion regulating molecule 1
NCBI Official Symbol
ADRM1
NCBI Official Synonym Symbols
ARM1; ARM-1; GP110
UniProt Protein Name
Proteasomal ubiquitin receptor ADRM1
UniProt Synonym Protein Names
110 kDa cell membrane glycoprotein; Gp110; Adhesion-regulating molecule 1; ARM-1; Proteasome regulatory particle non-ATPase 13; hRpn13; Rpn13 homolog
UniProt Gene Name
ADRM1
UniProt Synonym Gene Names
GP110; Gp110; ARM-1; hRpn13
NCBI Protein Information
proteasomal ubiquitin receptor ADRM1

NCBI Summary for ADRM1

This gene encodes a member of the adhesion regulating molecule 1 protein family. The encoded protein is a component of the proteasome where it acts as a ubiquitin receptor and recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Increased levels of the encoded protein are associated with increased cell adhesion, which is likely an indirect effect of this intracellular protein. Dysregulation of this gene has been implicated in carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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UniProt Comments for ADRM1

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. Within the complex, functions as a proteasomal ubiquitin receptor. Engages and activates 19S-associated deubiquitinases UCHL5 and PSMD14 during protein degradation. UCHL5 reversibly associate with the 19S regulatory particle whereas PSMD14 is an intrinsic subunit of the proteasome lid subcomplex.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ADRM1 antibody

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