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NCS1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200448

NCS1 is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. This protein regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for… More details

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Product Overview

Online Datasheet in PDF
Product Name
NCS1, Monoclonal Antibody
Full Product Name
NCS1 antibody
Product Gene Name
Product Synonym Names
Neuronal calcium sensor 1; Frequenin homolog; FLuP; FREQ; Neuronal calcium sensor 1 Flup; Freq; frequenin (Drosophila) homolog; Frequenin-like protein; NCS1; Neuronal Calcium Sensor 1.
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT11B8
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
19,728 Da
OMIM
X84048 mRNA
3D Structure
ModBase 3D Structure for P62166
Antigen Species
Human
Immunogen
Recombinant human NCS1 (1-190aa) purified from E. coli
Clone Note
Anti-human NCS1 mAb, clone AT11B8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NCS1 protein.
Applications Tested/Suitable for anti-NCS1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)

Western Blot (WB) of anti-NCS1 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: K562 cell lysate Lane 3.: LaCap cell lysate Lane 4.: HeLa cell lysate
Mouse Anti-NCS1 Monoclonal Antibody – Cat# MBS200448

Product References and Citations for anti-NCS1 antibody

Nakamura TY., et al (2001) Proc. Natl. Acad. Sci. u.S.A. 98: 12808-12813. ; Bahi N., et al (2003) Hum. Mol. Genet. 12: 1415-1425.

Other Notes

Small volumes of anti-NCS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NCS1 purchase

MBS200448 is an antibody targeting NCS1. Also known as NCS1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NCS1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neuronal calcium sensor 1 isoform 1
NCBI Official Synonym Full Names
neuronal calcium sensor 1
NCBI Official Symbol
NCS1
NCBI Official Synonym Symbols
FLUP; FREQ
UniProt Protein Name
Neuronal calcium sensor 1
UniProt Synonym Protein Names
Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein
UniProt Gene Name
NCS1
UniProt Synonym Gene Names
FLUP; FREQ; NCS-1
NCBI Protein Information
neuronal calcium sensor 1

NCBI Summary for NCS1

This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for NCS1

Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (). Stimulates PI4KB kinase activity (). Involved in long-term synaptic plasticity through its interaction with PICK1 (). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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