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HMOX Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200460

Heme oxygenase 1 belongs to the heme oxygenase family and is an essential enzyme in heme catabolism. It cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter.… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HMOX, Monoclonal Antibody
Full Product Name
HMOX antibody
Product Gene Name
Product Synonym Names
HO-1; Heat shock protein 32; HSP32; bK286B10; D8Wsu38e; Heme oxygenase (decycling) 1; Heme oxygenase 1; Hemox; Hmox; HMOX 1; HMOX1; HO; HO 1; HO1.
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT1D6
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (determined by BCA assay) (lot specific)
Preparation and Storage
Can be stored at 2-8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C
Avoid repeated freezing and thawing cycles
OMIM
X06985 mRNA
3D Structure
ModBase 3D Structure for P09601
Antigen Species
Human
Immunogen
Recombinant human Heme oxygenase1 (1-266aa) purified from E. coli

Product Categories/Family for anti-HMOX antibody

Applications Tested/Suitable for anti-HMOX antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-HMOX antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMOX antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A549 cell lysate Lane 3.: 293T cell lysate Lane 4.: HepG2 cell lysate Lane 5.: TF-1 cell lysate
Mouse Anti-HMOX Monoclonal Antibody – Cat# MBS200460

Immunofluorescence (IF) of anti-HMOX antibody

ICC/IF analysis of HMOX in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) ICC/IF analysis of HMOX in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)
Mouse Anti-HMOX Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200460

Flow Cytometry (FC) of anti-HMOX antibody

Flow cytometry analysis of HMOX in HeLa cells. The cell was stained with at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)
Mouse Anti-HMOX Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS200460

Product References and Citations for anti-HMOX antibody

Soares MP., et al. (2001) Immunol Rev.; Vareille M., et al. (2008) J Immunol. 180 (8): 5720-6.

Other Notes

Small volumes of anti-HMOX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HMOX purchase

MBS200460 is an antibody targeting HMOX. Also known as HMOX antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HMOX. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
heme oxygenase 1
NCBI Official Synonym Full Names
heme oxygenase 1
NCBI Official Symbol
HMOX1
NCBI Official Synonym Symbols
HO-1; HSP32; HMOX1D; bK286B10
UniProt Protein Name
Heme oxygenase 1
UniProt Gene Name
HMOX1
UniProt Synonym Gene Names
HO; HO1; HO-1
NCBI Protein Information
heme oxygenase 1

NCBI Summary for HMOX

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
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UniProt Comments for HMOX

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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