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BPGM Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200461

Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2,3-BPG concentration. It is responsible for the catalytic… More details

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Product Overview

Online Datasheet in PDF
Product Name
BPGM, Monoclonal Antibody
Full Product Name
BPGM antibody
Product Gene Name
Product Synonym Names
Bisphosphoglycerate mutase; BPG-dependent PGAM; Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase; erythrocyte; 2,3-bisphosphoglycerate mutase; 2,3-bisphosphoglycerate synthase; Ab2 098; AI323730 AL022789; BPG dependent PGAM; C86192.
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT2E11
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
30,005 Da
OMIM
X04327 mRNA
3D Structure
ModBase 3D Structure for P07738
Antigen Species
Human
Immunogen
Recombinant human BPGM (1-259aa) purified from E. coli.
Clone Note
Anti-human BPGM mAb, clone AT2E11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BPGM protein.

Product Categories/Family for anti-BPGM antibody

Applications Tested/Suitable for anti-BPGM antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-BPGM antibody

The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BPGM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BPGM transfected 293T cell lysate
Mouse Anti-BPGM Monoclonal Antibody – Cat# MBS200461

Immunofluorescence (IF) of anti-BPGM antibody

ICC/IF analysis of BPGM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BPGM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-BPGM Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200461

Product References and Citations for anti-BPGM antibody

Pritlove DC., et al. (2006) Placenta. 27 (8): 924-7. ; Wang Y., et al. (2006) J. Biol. Chem. 281 (51): 39642-8.

Other Notes

Small volumes of anti-BPGM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BPGM purchase

MBS200461 is an antibody targeting BPGM. Also known as BPGM antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BPGM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
bisphosphoglycerate mutase
NCBI Official Synonym Full Names
bisphosphoglycerate mutase
NCBI Official Symbol
BPGM
NCBI Official Synonym Symbols
DPGM
UniProt Protein Name
Bisphosphoglycerate mutase
UniProt Synonym Protein Names
2,3-bisphosphoglycerate mutase, erythrocyte; 2,3-bisphosphoglycerate synthase (EC:5.4.2.11
UniProt Gene Name
BPGM
UniProt Synonym Gene Names
DPGM
NCBI Protein Information
bisphosphoglycerate mutase

NCBI Summary for BPGM

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]
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UniProt Comments for BPGM

Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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