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ACADS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200465

ACADS (Acyl-Coenzyme A dehydrogenase), also known as SCAD or ACAD3, is a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ACADS, Monoclonal Antibody
Full Product Name
ACADS antibody
Product Gene Name
Product Synonym Names
Acyl-Coenzyme A dehydrogenase; ACAD3; SCAD; Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase; C2 to C3 short chain; Acyl-CoA dehydrogenase; C2 to C3 short chain; Acyl-CoA dehydrogenase; short chain; Acyl-Coenzyme A dehydrogenase; short chain; AI196007; Bcd-1; Bcd1; Butyryl CoA dehydrogenase; EC 1.3.99.2; Short chain acyl CoA dehydrogenase; unsaturated acyl CoA reductase; Short-chain specific acyl-CoA dehydrogenase; mitochondrial.
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT8B10
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
44,297 Da
OMIM
M26393 mRNA
Antigen Species
Human
Immunogen
Recombinant human ACADS (25-412aa) purified from E. coli
Clone Note
Anti-human ACADS mAb, clone AT8B10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACADS protein.

Product Categories/Family for anti-ACADS antibody

Applications Tested/Suitable for anti-ACADS antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-ACADS antibody

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACADS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : LnCap cell lysate
Mouse Anti-ACADS Monoclonal Antibody – Cat# MBS200465

Immunofluorescence (IF) of anti-ACADS antibody

ICC/IF analysis of ACADS in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACADS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ACADS in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACADS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-ACADS Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200465

Product References and Citations for anti-ACADS antibody

McAndrew RP., et al. (2008) J Biol Chem. 283 (14): 9435-43. ; Tafti M., et al. (2003) Nat Genet. 34 (3): 320-5.

Other Notes

Small volumes of anti-ACADS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACADS purchase

MBS200465 is an antibody targeting ACADS. Also known as ACADS antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ACADS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
NCBI Official Synonym Full Names
acyl-CoA dehydrogenase short chain
NCBI Official Symbol
ACADS
NCBI Official Synonym Symbols
SCAD; ACAD3
UniProt Protein Name
Short-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Synonym Protein Names
Butyryl-CoA dehydrogenase
UniProt Gene Name
ACADS
UniProt Synonym Gene Names
SCAD
NCBI Protein Information
short-chain specific acyl-CoA dehydrogenase, mitochondrial

NCBI Summary for ACADS

This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014]
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UniProt Comments for ACADS

Introduces a double bond at position 2 in saturated acyl-CoA's of short chain length, i.e. less than 6 carbon atoms.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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