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CSK Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200469

CSK is a tyrosine-protein kinase CSK that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by… More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CSK, Monoclonal Antibody
Full Product Name
CSK antibody
Product Gene Name
Product Synonym Names
Tyrosine-protein kinase CSK; Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL; Tyrosine protein kinase CSK
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT7C6
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
50,704 Da
OMIM
X60114 mRNA
3D Structure
ModBase 3D Structure for P41240
Antigen Species
Human
Immunogen
Recombinant human CSK (1-450aa) purified from E. coli
Clone Note
Anti-human CSK mAb, clone AT7C6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CSK protein.

Product Categories/Family for anti-CSK antibody

Applications Tested/Suitable for anti-CSK antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-CSK antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: CSK transfected 293T cell lysate
Mouse Anti-CSK Monoclonal Antibody – Cat# MBS200469

Immunofluorescence (IF) of anti-CSK antibody

ICC/IF analysis of CSK in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CSK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)
Mouse Anti-CSK Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200469

Product References and Citations for anti-CSK antibody

Braeuninger A., et al. (1991) Proc. Natl. Acad. Sci. U.S.A. 88: 10411-10415.

Other Notes

Small volumes of anti-CSK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CSK purchase

MBS200469 is an antibody targeting CSK. Also known as CSK antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CSK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tyrosine-protein kinase CSK
NCBI Official Synonym Full Names
C-terminal Src kinase
NCBI Official Symbol
CSK
UniProt Protein Name
Tyrosine-protein kinase CSK
UniProt Synonym Protein Names
C-Src kinase; Protein-tyrosine kinase CYL
UniProt Gene Name
CSK
NCBI Protein Information
tyrosine-protein kinase CSK

NCBI Summary for CSK

The protein encoded by this gene is involved in multiple pathways, including the regulation of Src family kinases. It plays an important role in T-cell activation through its association with the protein encoded by the protein tyrosine phosphatase, non-receptor type 22 (PTPN22) gene. This protein also phosphorylates C-terminal tyrosine residues on multiple substrates, including the protein encoded by the SRC proto-oncogene, non-receptor tyrosine kinase gene. Phosphorylation suppresses the kinase activity of the Src family tyrosine kinases. An intronic polymorphism (rs34933034) in this gene has been found to affect B-cell activation and is associated with systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]
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UniProt Comments for CSK

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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