Mouse Anti-PPIE Monoclonal Antibody – Cat# MBS200471

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPIE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate Lane 5.: K562 cell lysate Lane 6.: MCF7 cell lysate Lane 7.: LnCap cell lysate

PPIE Antibody – Mouse Monoclonal

Name: PPIE Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS200471
Price: 285 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200471

Cyclophilins possess the activity of peptidyl-prolyl cis-trans isomerase (PPIase) and are involved in cellular protein folding and protein interactions. PPIE contains two RNA binding domains at the N-terminal region and a PPIase domain at the C-terminal… More details

$285 / 0.05 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PPIE, Monoclonal Antibody

Full Product Name

PPIE antibody

Product Gene Name

anti-PPIE antibody

Product Synonym Names

Peptidylprolyl isomerase E isoform 1; CYP-33; Cyclophilin E; PPIase E; Rotamase E; peptidylprolyl isomerase E (cyclophilin E); PPIE; Peptidylprolyl isomerase E isoform 1 Cyclophilin 33; CYP33; EC 5.2.1.8 Peptidyl prolyl cis trans isomerase E

Clonality

Monoclonal

Isotype

IgG1, k

Clone Number

AT17E8

Host

Mouse

Species Reactivity

Human

Form/Format

Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

Purity/Purification

By protein-A affinity chromatography

Concentration

1 mg/ml (lot specific)

Preparation and Storage

Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

34,991 Da

OMIM

AF104012 mRNA

3D Structure

ModBase 3D Structure for Q9UNP9

Antigen Species

Human

Immunogen

Recombinant human PPIE (1-301aa) purified from E. coli

Clone Note

Anti-human PPIE mAb, clone AT17E8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPIE protein.

Product Categories/Family for anti-PPIE antibody

Epigenomics (Translation)

Applications Tested/Suitable for anti-PPIE antibody

ELISA (EIA), Western Blot (WB)

Product References and Citations for anti-PPIE antibody

Wang Y., et al. (2008) Genomics. FEBS Lett 582 (5): 835-9.; Mi H S., et al. (1996) FEBS Lett. 398: 201-5.

Other Notes

Small volumes of anti-PPIE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPIE purchase

MBS200471 is an antibody targeting PPIE. Also known as PPIE antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PPIE. It may not necessarily be applicable to this product.

NCBI GI #

5174637

NCBI GeneID

10450

NCBI Accession #

NP_006103

UniProt Primary Accession #

Q9UNP9

UniProt Secondary Accession #

O43634; O43635; Q32Q72; Q3S611; Q5TGA0; Q5TGA2; Q5TGA3; Q9UIZ5; B2R971

UniProt Related Accession #

Q9UNP9

GenBank Nucleotide #

NM_006112.3

NCBI Official Full Name

peptidyl-prolyl cis-trans isomerase E isoform 1

NCBI Official Synonym Full Names

peptidylprolyl isomerase E

NCBI Official Symbol

PPIE

NCBI Official Synonym Symbols

CYP33; CYP-33

UniProt Protein Name

Peptidyl-prolyl cis-trans isomerase E

UniProt Synonym Protein Names

Cyclophilin E; Cyclophilin-33; Rotamase E

UniProt Gene Name

PPIE

UniProt Synonym Gene Names

CYP33; PPIase E

NCBI Protein Information

peptidyl-prolyl cis-trans isomerase E

NCBI Summary for PPIE

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified. [provided by RefSeq, Aug 2010]

UniProt Comments for PPIE

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Combines RNA-binding and PPIase activities. May be involved in muscle- and brain-specific processes. May be involved in pre-mRNA splicing.

Research Articles on PPIE

1. CypE is a host restriction factor that inhibits the functions of nucleoprotein, as well as viral replication and transcription, by impairing the formation of the viral ribonucleoprotein complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PPIE antibody

Products by Pathway	Pathway Diagram
DNA Repair Pathway antibodies	DNA Repair Pathway Diagram
Dual Incision In TC-NER Pathway antibodies	Dual Incision In TC-NER Pathway Diagram
Formation Of TC-NER Pre-Incision Complex Pathway antibodies	Formation Of TC-NER Pre-Incision Complex Pathway Diagram
Gap-filling DNA Repair Synthesis And Ligation In TC-NER Pathway antibodies	Gap-filling DNA Repair Synthesis And Ligation In TC-NER Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Neutrophil Degranulation Pathway antibodies	Neutrophil Degranulation Pathway Diagram
Nucleotide Excision Repair Pathway antibodies	Nucleotide Excision Repair Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram

Diseases associated with anti-PPIE antibody

Disease Name	Pubmed Publications
Abnormalities, Multiple Antibodies	>3 publications with PPIE and Abnormalities, Multiple
Nervous System Diseases Antibodies	>3 publications with PPIE and Nervous System Diseases
Premature Birth Antibodies	>1 publications with PPIE and Premature Birth
Neoplasms, Experimental Antibodies	>1 publications with PPIE and Neoplasms, Experimental
Cardiovascular Diseases Antibodies	>1 publications with PPIE and Cardiovascular Diseases
Precancerous Conditions Antibodies	>1 publications with PPIE and Precancerous Conditions
Adenocarcinoma Antibodies	>1 publications with PPIE and Adenocarcinoma
Hyperplasia Antibodies	>1 publications with PPIE and Hyperplasia
Hypertension Antibodies	>1 publications with PPIE and Hypertension
Necrosis Antibodies	>1 publications with PPIE and Necrosis

Organs/Tissues associated with anti-PPIE antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>4 publications with PPIE and Lung
Brain Antibodies	>4 publications with PPIE and Brain
Blood Antibodies	>3 publications with PPIE and Blood
Kidney Antibodies	>2 publications with PPIE and Kidney
Esophagus Antibodies	>1 publications with PPIE and Esophagus
Muscle Antibodies	>1 publications with PPIE and Muscle
Skin Antibodies	>1 publications with PPIE and Skin