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OTUB2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200474

OTUB2 (Ubiquitin thioesterase OTUB2) belongs to the peptidase C65 family. OTUB2 functions as a hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
OTUB2, Monoclonal Antibody
Full Product Name
OTUB2
Product Gene Name
Product Synonym Names
ubiquitin thioesterase OTuB2; OTB2; OTu2
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
AT1F8
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid; In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Purity/Purification
Protein-A Affinity Chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
OMIM
608338
3D Structure
ModBase 3D Structure for Q96DC9
Immunogen
Recombinant human OTUB2 (1-234aa) purified from E Coli.
Conjugation
Unconjugated

Product Categories/Family for anti-OTUB2 antibody

Applications Tested/Suitable for anti-OTUB2 antibody
ELISA (EIA), Western Blot (WB)

Western Blot (WB) of anti-OTUB2 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OTUB2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: OTUB2 transfected 293T cell lysate
Mouse Anti-OTUB2 Monoclonal Antibody – Cat# MBS200474

Product References and Citations for anti-OTUB2 antibody

Balakirev MY, et al. (2003) EMBO Rep 4 (5): 517-22. Edelmann M.J., et al. (2009) Biochem. J. 418:379-390.

Other Notes

Small volumes of anti-OTUB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OTUB2 purchase

MBS200474 is an antibody targeting OTUB2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for OTUB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin thioesterase OTUB2
NCBI Official Synonym Full Names
OTU deubiquitinase, ubiquitin aldehyde binding 2
NCBI Official Symbol
OTUB2
NCBI Official Synonym Symbols
OTB2; OTU2; C14orf137
UniProt Protein Name
Ubiquitin thioesterase OTUB2
UniProt Synonym Protein Names
Deubiquitinating enzyme OTUB2; OTU domain-containing ubiquitin aldehyde-binding protein 2; Otubain-2; Ubiquitin-specific-processing protease OTUB2
UniProt Gene Name
OTUB2
UniProt Synonym Gene Names
C14orf137; OTB2; OTU2
UniProt Entry Name
OTUB2_HUMAN
NCBI Protein Information
ubiquitin thioesterase OTUB2

NCBI Summary for OTUB2

This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. [provided by RefSeq, Aug 2011]
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UniProt Comments for OTUB2

OTUB2: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. Belongs to the peptidase C65 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Hydrolase; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: nucleus

Molecular Function: ubiquitin-specific protease activity

Biological Process: protein deubiquitination; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-OTUB2 antibody

Organs/Tissues associated with anti-OTUB2 antibody

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